益生菌对大鼠肝脏脂质过氧化损伤拮抗作用的研究

Investigation on Effect of Antagonism of Lipid Peroxidation to Liver by Probiotics in Rats

研究益生菌对大鼠肝脏脂质氧化损伤拮抗作用。40只SPF级SD雄性大鼠通过高脂饮食建立脂质过氧化损伤模型,随机分成空白组、模型组、益生菌LV108组和益生菌混合组。空白组给予普通饲料,其余各组给予高脂饲料饲养,同时两组益生菌干预组分别灌胃LV108发酵乳和混合发酵乳(1 m L/100 g,109 CFU/m L)。结果表明,各组间大鼠饲料消耗无显著性差异,第4周后益生菌组及其混合发酵乳组体重增量显著低于模型组。大鼠血清指标表明益生菌干预能够降低血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)水平,提高高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量。大鼠肝脏氧化指标可以看出,益生菌干预能够提高抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化氢酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase from micrococcus lysodeikticus,CAT)的活力和非酶抗氧化物质谷胱甘肽(glutathione,GSH)的含量,清除超氧阴离子自由基、羟自由基、一氧化氮自由基(NO)等自由基,减少脂质过氧化物丙二醛(malondialdehyde,MDA)的含量。益生菌对大鼠脂质过氧化损伤拮抗作用通过降低高脂饲料的食物效应比,缓解脂质堆积,调控血脂及肝脏脂质氧化损伤来实现。

The effect of antagonism of lipid peroxidation to liver by probiotics in rats was studied.A model of lipid peroxidation was established by high-fat diet. Forty male SD rats were randomly divided into blank group,model group,probiotic LV108 group and probiotic mixed group.The blank group was treated with normal feed,and the rest groups were treated with high fat diet. At the same time,probiotic LV108 group and probiotic mixed group were given LV108 fermented milk and mixed fermented milk（1 mL/100 g,109 CFU/mL）respectively.The results showed that there was no significant difference in food intake between the groups. After the fourth week,the weight gain of the probiotic group and the mixed fermented milk group was significantly lower than that of the model group.Serum indicators show that probiotics could reduce triglyceride（TG）,total cholesterol（TC）and low density lipoprotein cholesterol（LDL-C）,increase high density lipoprotein cholesterol（HDL-C）.It can be seen from the rats liver oxidation index that the probiotics could increase the activity of antioxidant enzymes superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px）, catalase from micrococcus lysodeikticus（CAT）and the content of non-enzymatic antioxidant glutathione（GSH）,and eliminate the superoxide anion free radical,hydroxyl free radical,NO free radical and other free radicals,reduce lipid peroxide（MDA）.We speculate that the effect of antagonism of lipid peroxidation to liver by probiotics in rats may be achieved by reducing the food-effect ratio of high-fat diet,relieve lipid accumulation,regulate lipid and liver lipid oxidation damage.