高流体静力压下脾静脉和大隐静脉基质金属蛋白酶及抑制物失调研究

Imbalance in matrix metalloproteinases and matrix metalloproteinase inhibitors from diseased splenic vein and varicose great saphenous vein under high hemodynamics

目的:探讨高流体静力压下脾静脉和大隐静脉管壁基质金属蛋白酶及抑制物的差异。方法:收集病脾静脉(DSVs)、正常脾静脉(NSVs)、曲张大隐静脉(VGSVs)、正常大隐静脉(NGSVs)标本72份。观察4组静脉管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白表达。检测4组静脉管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达比率和m RNA表达。结果:在DSVs和VGSVs管壁中,MMP-2、MMP-9、TIMP-1、TIMP-2蛋白呈簇状强阳性表达。DSVs组管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达比率和m RNA表达与NSVs相比显著增多,差异有统计学意义(P<0.05);VGSVs组与NGSVs组相比显著增多,差异有统计学意义(P<0.05);VGSVs/NGSVs比值与DSVs/NSVs比值相比显著增高,差异亦有统计学意义(P<0.05)。结论:高流体静力压下,脾静脉和大隐静脉管壁基质金属蛋白酶及抑制物平衡失调,这可能是静脉管壁重塑的分子机制之一。

Objective： To investigate the differences in matrix metalloproteinases （MMPs） andmatrix metalloproteinase inhibitors （TIMPs） from diseased splenic vein （DSVs） and varicose greatsaphenous vein （VGSVs） under high hemodynamics. Methods： Seventy-two specimens of DSVs,normal splenic veins （SVs）, VGSVs, and normal great saphenous vein （GSVs） were collected. Venouswall in the four groups, MMP-2, MMP-9, TIMP-1, and TIMP-2 protein expression were observedand MMP-2, MMP-9, TIMP-1, TIMP-2 proteins positive expression ratio and mRNA expressionwere determined. Results： DSVs and VGSVs in the two groups, MMP-2, MMP-9, TIMP-1, TIMP-2proteins with clustered strong expression were observed; In DSVs group, MMP-2, MMP-9, TIMP-1,TIMP-2 protein positive expression ratio andmRNA expression were significantly increased comparedwith SVs group, while in VGSVs group, MMP-2, MMP-9, TIMP-1, TIMP-2 protein positive expressionratio and mRNA expression were significantly increased compared with GSVs group （ 〈0.05）.VGSVs/GSVs ratio was significantly increased compared with DSVs/SVs ratio （ 〈0.05）. Conclusion：Under high hemodynamics, the dysequilibrium of MMPs and TIMPs from splenic vein and greatsaphenous vein, These results may be one of themolecularmechanismin vascular remodeling.