双孢蘑菇液体培养基筛选及培养条件优化

Optimization of the Screening and Culture Conditions of the Liquefied Spawn of Agaricus bisporus

目前国内双孢蘑菇生产多采用固体菌种进行栽培,但固体菌种在生产过程中存在生长周期长、效率低等问题。因此,本文采用正交试验方法,以双孢蘑菇菌丝体的生物量为测量指标,优化液体菌种的培养基配方及其培养条件,促进菌丝生长,在短期内使菌丝体的生物量达到最大,弥补了固体菌种在生产中的不足。试验结果显示,双孢蘑菇的最佳培养基配方为玉米淀粉3%,蔗糖2%,酵母粉0.5%,磷酸二氢钾0.15%,硫酸镁0.2%;最适培养条件为温度23℃,pH 5,转速200 r/min,250 mL三角瓶中的最适装液量为150 mL。

At present, the solid spawn is widely used in the cultivation of Agaricus bisporus in China. However, the growthcycle of solid spawn is very long and the efficient is low. Therefore, the orthogonal test method was used in this paper, takingthe biomass of mycelium of Agaricus bisporus as the measurement index, the medium formulation and culture conditions ofliquefied spawn were optimized to promote the growth of mycelium, the biomass of mycelium was maximized in a short time,this made up for the deficiency of solid spawn in production. The results illustrated the best medium formula was composedof 3% corn starch, 2% sucrose, 0.5% yeast powder, 0.15% potassium dihydrogen phosphate and 0.2% magnesium sulfate.The optimum culture conditions were as follows： temperature 23 ℃, pH 5, rotational speed 200 r / min, the optimal volumeof liquid in 250ml triangular bottle 150 mL.