慢性萎缩性胃炎患者Hp感染与TGF-βRⅡ、IL-6和TNF-α的表达研究

Hp infection and expression of TGF-βR Ⅱ,IL-6 and TNF-α in patients with chronic atrophic gastritis

目的:探讨慢性萎缩性胃炎患者幽门螺旋杆菌感染与胃黏膜中转化生长因子β受体Ⅱ、白介素6和肿瘤坏死因子α的表达情况。方法:选取76例慢性萎缩性胃炎(CAG)患者胃黏膜病变组织的胃镜活检标本,采取PCR荧光法检测Hp感染情况,免疫组化法检测TGF-βRⅡ、IL-6和TNF-α在胃小凹上皮和间质炎细胞中的表达。结果:慢性炎症程度在Hp感染阳性组和阴性组中差异有统计学意义(P<0.05);间质炎细胞中IL-6的表达在Hp感染阳性组和阴性组中差异有统计学意义(P<0.05);TGF-βRⅡ、TNF-α的表达在Hp感染阳性组和阴性组中无显著性差异(P>0.05);间质炎细胞中IL-6与慢性炎症程度呈正相关关系(r=0.249,P=0.03);萎缩程度与肠上皮化生严重程度、上皮内瘤变程度呈正相关关系(r=0.697,0.366)。结论:IL-6在间质炎性细胞中的表达与Hp相关性CAG患者的慢性炎症程度有关,慢性萎缩程度促进肠上皮化生和上皮内瘤变的发生。

Objective： To investigate the effect of Helicobacter pylori（ Hp） infection in patients with chronic atrophic gastritis（ CAG） and the expression of transforming growth factor-β receptor Ⅱ,interleukin 6 and tumor necrosis factor-α in gastric mucosa. Methods： 76 cases of gastroscope biopsy specimens were collected in the CAG patients,the infection of Hp was detected by PCR fluorescence,and immunohistochemical staining was used to determine the expression of TGF-βRⅡ,IL-6 and TNF-α in gastric foveolar epithelium and stromal inflammatory cells. Results： There were significant differences in chronic inflammation between Hp-positive and Hp-negative group（ P〈0. 05）. Expression of IL-6 in stromal inflammatory cells was significantly different between Hp-positive and Hp-negative group（ P〈0. 05）. Expression of TGF-βRⅡ and TNF-α was not significantly different in two groups（ P〉0. 05）. IL-6 expressed instromal inflammatory cells were correlated with chronic inflammation（ r = 0. 249,P = 0. 03）. The degree of intestinal metaplasia and dysplasia were correlated with the atrophic severity respectively（ r = 0. 697,0. 366）. Conclusion： IL-6 is related to the chronic inflammation in patients with CAG who has Hp infection. The chronic atroplic severity promotes the development of intestinal metaplasia and dysplasia.