支气管肺泡灌洗液MGMT基因甲基化检测在非小细胞肺癌诊断中的价值

Values of promoter hypermethylation of MGMT gene in Bronchoalveolar lavage fluid on the diagnosis of non-small cell lung cancer

目的探讨支气管肺泡灌洗液(BALF)中MGMT基因启动子甲基化水平对于非小细胞肺癌的诊断价值。方法选取完成胸部手术患者98例为研究对象,经病理确诊非小细胞肺癌患者63例组成试验组,经病理确认为良性病变患者35例为对照组,分别获取试验组及对照组成员血清标本、BALF标本及活组织标本,对不同类型标本中MGMT基因启动子甲基化水平做检测。结果非小细胞肺癌患者肺癌组织中、BALF中、血清中MGMT基因甲基化发生率分别为58.7%、52.4%和27%;肺癌组织的MGMT基因甲基化率与BALF中MGMT基因的甲基化率有较好的一致性,其与血清标本中MGMT基因的甲基化一致性较差,其判断肿瘤良恶性的敏感度低于BALF标本;35例肺良性病变对照组的肺组织标本、BALF及血清标本,均未检测到MGMT基因甲基化表达,其甲基化率为0。结论 BALF中MGMT基因甲基化检测在非小细胞肺癌的筛查诊断中具有一定的临床应用价值。

Objective To discuss the diagnostic value of the promoter hypermethylation of MGMT gene of Bronchoalveolar lavage fluid（BALF）on non-small cell lung cancer.Methods 98 patients underwent chest surgery were selected as study subjects;63 patients,pathologically diagnosed as non-small cell lung cancer,were enrolled in this study.35 patients with benign lesions were selected as the control group.Serum samples,BALF specimens and biopsy specimens were taken from different groups;MGMT gene promoter methylation level for the specimens was detected.Results The positive rates of promoter methylation of MGMT gene in lung cancer tissues,BALF specimens and the serum samples was 58.7% ,52.4% and 27% ,respectively.The positive rates of promoter methylation of MGMT gene in lung cancer tissues and the values of BALF specimens coincided with each other.While the consistency of promoter methylation of MGMT gene with lung cancer tissues and that of serum specimens is poor.The sensitivity of benign or malignant tumors detection was lower than that of BALF specimens.In the group of 35 patients with benign lesions,the methylation of the MGMT gen was not detected in the biopsy specimens,the BALF specimens and the serum samples;the incidence of methylation was 0.Conclusion Methylation detection of MGMT gene in BALF had certain clinical application value in the diagnosis of non-small cell lung cancer.