胎儿生长受限母胎界面HIF-1α的表达及意义研究

A study on the expression and significance of HIF-1α on the maternal-fetal interface of fetal growth restriction

目的对照研究正常足月新生儿和FGR患儿母胎界面HIF-1α表达,探讨HIF-1α及其靶基因在FGR发病机制中的作用。方法采用经典ELISA法检测两组母血、脐血、胎盘母面及子面组织匀线计算各种物质的含量;对两组多聚甲醛固定的胎盘组织经石蜡包埋浆中的HIF-1α含量进行对照分析,检测结果是根据标准曲线计算HIF-1α的含量,组织标本切片后进行免疫组化染色及荧光染色后,检测HIF-1α在胎盘母面及子面的表达,显微镜观察组织形态变化,利用图像分析仪对显示强度进行定量分析;通过电子显微镜观察两组标本细胞内组织结构的变化,并进行分析。结果免疫组化染色和免疫荧光染色均显示FGR胎盘子面强阳性。ELISA检测结果:FGR胎盘母面与正常足月儿胎盘母面比较,两者差别没有统计学意义;FGR胎盘子面与正常足月儿胎盘子面比较,两者差别有统计学意义;FGR母血与正常足月儿母血比较,两者差别没有统计学意义;FGR脐带血与正常足月儿脐带血比较,两者差别有统计学意义。结论 FGR胎盘子面表达及脐血中HIF-1α水平明显升高,通过诱发血管内皮损伤减少胎盘子面血流量,影响胎儿血液供应,出现慢性缺氧,在FGR发病机制中起重要作用。

Objective To study the expression of HIF-1αon maternal fetal interface between normal full-term newborns and fetal growth restriction（FGR）fetus and discuss the effect of HIF-1αand its target gene in the pathogenesis of FGR.MethodsThis study used classic ELISA method to detect the content of various substances in maternal blood,umbilical cord blood,cmaternal and child tissue surface of two groups by uniform linear calculation.Conducted a comparative analysis of the content of HIF-1αin poly formaldehyde fixed and wax embedded placental tissue of two groups,the test results were based on the content of HIF-1αcalculated by standard curve,after the tissue sections being immunohistochemical stained and fluorescence stained,examine the expression of HIF-1αon the maternal surface and child surface.Microscope was used to observe the histological changes and the display intensity was determined by quantitative analysis by the image analyzer.The changes of inner organization structure in two sample cells were observed through an electron microscope,and the followed analysis is based on above mentioned observations.Results Immunohistochemical stains and immunofluorescence staining showed strong positive on the sub surface of FGR placenta.Compared the maternal surface of FGR with the maternal surface of normal full-term new born,the difference had no statistical significance;compared the placenta sub surface of FGR with the placenta sub surface of normal full-term,the difference had statistical significance;compared the maternal blood of FGR with the maternal blood of normal fullterm fetus,the difference had nostatistical significance;compared the umbilical cord blood of FGR with the umbilical cord blood of normal full-term one,the difference had statistical significance.Conclusion The expression of HIF-1αon FGR placenta sub surface and the level of umbilical cord blood are significantly increasing by reducing the blood flow of the sub surface of placenta via inducing vascular endothelial injury,posing impact on the blood supply to the fetus,and induce chronic hypoxia,which plays an important role in the pathogenesis of FGR.