基于光学微血流成像技术的相干光断层扫描血流成像对视网膜血流定量分析的可重复性评价

Repeatability of quantitative assessment of the retinal microvasculature using optical coherence tomography angiography based on optical microangiography

目的评价基于光学微血流成像技术(OMAG)的相干光断层扫描血流成像(OCTA)在正常眼视网膜血流定量分析的可重复性。设计诊断试验。研究对象 40名(40眼)健康志愿者,年龄22~55岁,平均(33.0±8.8)岁。方法使用Zeiss Cirrus HD-OCT 5000扫描,以基于OMAG技术的AngioPlex^(TM)软件对视网膜浅层3 mm×3 mm OCTA图像的血流长度密度(VLD)、灌注密度(PD)及黄斑中心凹无血流信号区(FAZ)面积、周长、圆形度进行自动定量分析。采用变异系数(CV)评价观察日内及观察日间重复性,组内相关系数(ICC)评价观察者间再现性。主要指标变异系数(CV)和组内相关系数(ICC)。结果各血流参数观察日内CV(1.2±1.0)%^(4.2±5.5)%,均≤4.2%,观察日间CV(1.4±0.8)%^(4.6±4.9)%,均≤4.6%,观察日间CV高于观察日内CV,而中心凹、FAZ各参数CV高于旁中心凹CV;各血流参数观察者间ICC(0.923~0.994),均≥0.923,中心凹、FAZ各参数ICC高于旁中心凹。结论基于OMAG技术的OCTA对正常眼视网膜浅层血流各参数测量均具有较好的重复性和再现性。

Objective To determine the repeatability of optical coherence tomography angiography（ OCTA） based on optical microangiography（ OMAG） measurements of macular vessels in normal eyes. Design Diagnosis test. Participants A total of 40 eyes of 40 normal volunteers were examined at Beijing Tongren Eye Center. The mean age was 33. 0 ± 8. 8 years（ 22 ~ 55 years）. Methods OCTA data was acquired using Zeiss Cirrus HD-OCT 5000 angiography system. The following parameters were used for quantitative analysis： vessel length density（ VLD）,perfusion density（ PD） and area,perimeter,circularity of foveal avascular zone（ FAZ）. Main Outcome Measures The coefficient of variation（ CV） and intraclass correlation coefficients（ ICC）. Results All parameters showed high repeatability [CV（ 1. 2 ± 1. 0） % ^（ 4. 2 ±5. 5） %,all ≤4. 2%（ intravisit） and（ 1. 4 ± 0. 8） % ^（ 4. 6 ± 4. 9） %,all≤4. 6%（ intervisit） ] and interobserver reproducibility（ ICC = 0. 923 ~ 0. 994,all≥0. 923）. The CVs were higher for intervisit than for intravisit,higher for fovea and FAZ than for parafovea. The ICCs were higher for fovea and FAZ than for parafovea. Conclusion The evidence presented here demonstrates good repeatability and reproducibility of OCTA based on OMAG for measurement of superficial vessel parameters in normal eyes.