槲寄生预防围绝经期雌兔干眼的实验研究

Experimental study on mistletoe in prevention of perimenopausal xerophthalmia in female rabbits

目的观察槲寄生滴眼液对预防围绝经期雌兔干眼的效果。设计实验研究。研究对象健康雌兔36只。方法取24只雌兔行双侧卵巢切除术,术后随机分为2组,其中12只为生理盐水滴眼液组(A组)、12只为槲寄生滴眼液组(B组)(均点右眼),连续滴眼2个月,每日4次。余12只雌兔(C组)打开腹腔,不切除卵巢,眼部不进行任何处理,作为假手术组。分别于干预前,干预后1周、2周、1个月及2个月对受试兔进行基础泪液分泌(SIT)检查、角膜荧光素染色(FL)、泪液蛋白测定及角膜共聚焦显微镜扫描。主要指标SIT、FL评分、泪液蛋白测定及角膜共聚焦显微镜扫描。结果干预2个月后,B组SIT(7.48±3.16)l/mm、FL评分(1.91±0.96)、总蛋白(10.71±2.37)ρ/g·L^(-1)、溶菌酶(1.43±0.35)ρ/g·L^(-1)、乳铁蛋(1.19±0.27)ρ/g·L^(-1)、淀粉酶活性(87.75±62.79)IU、角膜上皮下细胞密度(3174±379)cells/mm^2,均较A组改善(P均<0.05),角膜前基质发光细胞较C组密度稍有增多,角膜基质层神经干稍有弯曲。结论槲寄生滴眼液可有效改善围绝经期雌兔SIT、FL的评分和泪液蛋白、角膜上皮下细胞密度等指标。

Objective To investigate the effect of mistletoe eye drops in prevention of perimenopausal xerophthalmia in female rabbits. Design Experimental study. Participants Thirty-six healthy female rabbits. Methods Thirty-six healthy female rabbits were chosen,among them,24 female rabbits were cut off bilateral ovaries set up perimenopausal dry eye model. These 24 rabbits were randomly divided into 2 groups： group A was treated with normal saline eye drops,group B was treated with mistletoe eye drops. The other 12 rabbits（ group C）,as a control group,underwent sham surgery to open abdominal cavity,but their ovaries were not removed. Schirmer＇s test（ SIT）,corneal fluorescein（ FL） staining,tear proteins,and corneal confocal microscope were performed before intervention and at 1 week,2 weeks,1 month,2 months after intervention. Main Outcome Measures SIT,FL,tear proteins,and corneal confocal microscope.Results At 2 months after intervention,rabbits in group B showed significant better results of SIT（ 7. 48 ±3. 16） l/mm,FL score（ 1. 91 ± 0. 96）,total protein（ 10. 71 ± 2. 37） ρ/g·L^（-1）,lysozyme（ 1. 43 ± 0. 35）ρ/g·L^（-1）,iron egg milk（ 1. 19 ± 0. 27） ρ/g·L^（-1）,amylase activity（ 87. 75 ± 62. 79） IU,corneal epithelium cell density（ 3174 ± 379） cells/mm^2 and corneal anterior substrate light emitting cell density,compared to group A rabbits（ all P〈 0. 05）. Compared with the nornal rabbit,corneal anterior substrate light emithing cells desity slightly increased,and corneal stromal layer nerve trunk slightly curved. Conclusion Mistletoe eye drops can effectively improve SIT,the score of FL,lacrimal protein,corneal epithelial cell density and other indicators in the perimenopolis.