瓜拉纳多糖制备的响应面工艺优化及抗氧化活性研究

Optimization of Extraction of Polysaccharides from Paullinia Cupana by Response Surface Methodology and Study on Its Antioxidant Activities

利用响应面法优化瓜拉纳多糖的提取工艺。采用超声辅助水提法,在单因素试验的基础上,以提取时间、超声功率、液料比、提取温度为变量,以瓜拉纳多糖得率为响应值,应用Box-Benhken设计及响应面分析法进行回归分析,得到最佳提取工艺为提取时间33.5 min,超声功率155 W,水料体积质量比35 m L︰1 g,提取温度62℃,醇沉透析后冷冻干燥得到瓜拉纳多糖,此条件下瓜拉纳多糖得率为20.80%。对瓜拉纳多糖体外抗氧化活性进行评价,并以维生素C(Vc)作对照,结果发现,瓜拉纳多糖还原能力和总的抗氧化活性均与多糖浓度成正比,但都小于Vc,其中抗氧化能力接近Vc。

The extraction of polysaccharide from Paullinia Cupana was optimized by response surface methodology in this study. Based on the single-factor experiment, the extraction time, ultrasonic power, water/solid ratio and extraction temperature were used as variables, the yield of guarana polysaccharide was used as the response value, and the Box-Benhken design and response surface analysis method was applied for regression analysis, the optimum conditions obtained through RSM analysis were as follows： the extraction time 33.5 min, ultrasonic power 155 W, water/solid ratio（mL/g） of 35/1, extraction temperature 62 ℃, ethanol precipitation and freeze-dried treatments. Under the optimal conditions, the yield of guarana polysaccharide was 20.80%. The in vitro antioxidant activity of this polysaccharide was evaluated and Vc was used as a control. Its reduction activity and the total antioxidant activity were in a concentration-dependent manner, they were all less than that of Vc, but the antioxidant capacity of guarana polysaccharide closed to that of Vc.