结肠癌中长链非编码RNA表达谱分析

Study on expression profile of long non-coding RNA in colorectal carcinoma

目的建立结肠癌肿瘤组织与癌旁组织中长链非编码RNA（1ncRNA）的差异表达谱。方法分别提取2015年7月至8月于首都医科大学附属北京朝阳医院普外科行手术治疗的6例结肠癌患者肿瘤组织和癌旁组织中的RNA，并对总RNA中的mRNA进行放大、标记和纯化，与含有lncRNA的芯片进行杂交后，对数据进行归一化处理及差异分析。结果在肿瘤组织和癌旁组织芯片中，筛选出差异表达的lncRNA1339条（P〈0．05）。有明显差异（变化〉2倍）的lncRNA共53条，其中在肿瘤组织中高表达的lncRNA41条，低表达12条。变化在3倍以上的lncRNA共3条。结论结肠癌组织与癌旁组织比较，其lncRNA表达谱发生明显改变，提示lncRNA可能参与结肠癌的发生。

Objective To analyze the differentially expressed long non-cording RNA （lncRNA） in human colon carcinoma tissue and paracancerous normal colon tissue. Methods Total RNA from the colon carcinoma tissues and paracancerous normal colon tissues of 6 patients from July to August 2015 in Beijing Chao-Yang Hospital, Capital Medical University were prepared respectively. The mRNA of total RNA was amplified, labeled and purified. Hybridization was performed with the profile chip, and the data were normalized and analyzed. Results Compared with normal tissues, 1 339 lncRNAs expressed differentially （P 〈 0.05）, 53 lncRNAs were significantly differential （fold change 〉 2） in colon carcinoma tissues, in which 41 lncRNAs were up-regulation and 12 lncRNAs were down-regulation. Three lncRNAs had more than 3 times changes. Conclusion Obvious changes of lncRNA expression profile are observed in the pathogenesis of colon carcinoma, suggesting that lncRNA may be related to the progress of colon carcinoma.