参芪扶正注射液通过STAT3通路抑制人胃癌细胞SGC7901增殖的相关研究

Anti-proliferative effect of Shenqi Fuzheng injection on gastric cancer cell line SGC7901 via STAT3 pathway

目的 研究参芪扶正注射液（参芪液）对人胃癌细胞SGC7901增殖的影响,并探讨其与信号转导和转录活化因子3（STAT3）通路相关的作用机制.方法 以人胃癌细胞SGC7901为研究对象,采用四甲基偶氮唑盐（MTT）法观察不同浓度参芪液对SGC7901细胞活性的影响;采用酶联免疫吸附试验（ELISA）检测上清液中炎症因子白细胞介素（IL）-6、IL-8和IL-11的含量;采用蛋白印迹法检测细胞中Janus激酶2（JAK2）、STAT3和磷酸化STAT3（p-STAT3）的表达.结果 与空白对照组相比,当参芪液浓度达到0.1 mg/ml时,SGC7901细胞抑制率达到（10.8±0.7）%,差异具有统计学意义（P〈0.01）,随着参芪液浓度的逐渐增高,SGC7901细胞抑制率也逐渐增高（F=12.319,P=0.000）.与空白对照组相比,随着参芪液浓度的增加,SGC7901细胞中IL-6、IL-8和IL-11含量均呈下降趋势,且差异具有统计学意义（IL-6：F=31.256,P=0.000;IL-8：F=16.857,P=0.000;IL-11：F=21.319,P=0.000;各指标中各浓度组与空白对照组比较均P〈0.05）.与空白对照组相比,随着参芪液浓度的增加,JAK2和p-STAT3蛋白表达水平下调（JAK2：F=12.315,P=0.000;p-STAT3：F=16.728,P=0.000;各指标中各浓度组与对照组比较均P〈0.05）.结论 参芪扶正注射液能够抑制SGC7901细胞增殖,其机制可能与抑制STAT3通路有关.

Objective To study the effect of Shenqi Fuzheng injection on proliferation of gastric cancer cell line SGC7901, and to explore its mechanism associated with the STAT3 pathway. Methods The human gastric cancer cell line SGC7901 was used as the research object, the effects of different concentrations of Shenqi Fuzheng injection on the activity of SGC7901 cells were observed by MTT method, the levels of interleukin（IL）-6,IL-8 and IL-11 were detected by enzyme-linked immunosorbent assay（ELISA）, and the expressions of Janus kinase 2（JAK2）, STAT3 and phosphorylated-STAT3（p-STAT3） were detected by Western blot. Results Compared with the control group,the activity of SGC7901 cells in the treatment group decreased significantly, and the concentration of Shenqi Fuzheng injection reached 0.1 mg/ml, the inhibitory rate of SGC7901 cells reached （10.8±0.7） %, and the difference was statistically significant （P〈 0.01）. With the increase of Shenqi Fuzheng injection concentration, the inhibition rate of SGC7901 cells increased gradually （F =12.319, P =0.000）. Compared with the control group, with the increase of Shenqi Fuzheng injection concentration, IL-6, IL-8 and IL-11 contents were decreased in SGC7901 cells, the differences were statistically significant （IL-6： F = 31.256, P = 0.000; IL-8： F = 16.857, P = 0.000; IL-11： F = 21.319, P =0.000）;JAK2 and p-STAT3 protein levels were significantly reduced（JAK2：F =12.315,P =0.000;p-STAT3：F= 16.728, P= 0.000） in the treatment groups. Conclusion Shenqi Fuzheng injection can inhibit the proliferation of SGC7901 cells,and the mechanism may be related to the inhibition of STAT3 pathway.