外源转基因片段从转基因棉籽壳到食用菌水平转移研究

Study on the transferring of the gene fragment from the genetically modified cottonseed hulls to the edible fungi

研究利用转基因棉籽壳种植食用菌是否会导致外源转基因片段从棉籽壳水平转移到食用菌中。从银耳生产基地与菇厂采集15份银耳样品与10份平菇样品(培养基质中转基因棉籽壳含量分别约为75%与92%),用PCR扩增检测是否存在转基因棉籽壳中的外源片段CaMV 35S、NOS、CpTⅠ、Cry IA(c)。此外,针对可能会用含棉籽壳基质培养的市售食用菌,从上海市闵行区各大中型超市与农贸市场采集市售样品47份,用PCR扩增检测外源转基因片段。结果显示,不论是生产基地明确用转基因棉籽壳培养的银耳与平菇样品,还是市售的各类食用菌均未发现存在外源转基因片段向食用菌水平转移的现象。

It is a question in the field of food-safety that whether foreign genetically modified genes would transfer from the genetically modified cottonseed hulls to edible fungi.In this study,15 Tremella fuciformis samples and 10 Pleurotus ostreatus samples（the cultivation media contained 75% and 92% genetically modified cottonseed hulls,respectively）collected from cultivation meida were evaluated by PCR to confirm whether the foreign genetically modified genes,CaMV 35 S,NOS,CpTⅠ,Cry IA（c）,were present.In addition,47 samples which were probably grown in the media containing cottonseed hulls,were collected from the large and medium-sized supermarkets and farm produce markets.All of the results of PCR showed that the foreign sequences were not amplified by PCR.In conclusion,No exogenous transgenic fragments in the genetically modified cottonseed hulls was found transferred to the edible fungi.