摘要
高效液相色谱法因操作简单、高效和灵敏度高成为单糖分析的重要方法之一。文章介绍几种常用单糖分析的高效液相色谱方法,即液相色谱示差折光检测法、液相色谱蒸发光散射检测法、PMP柱前衍生化检测法、对氨基苯甲酸柱前衍生法、邻氨苯甲酸衍生法、柱后衍生法,指出它们的优缺点,并从灵敏度、操作方法、精确度等方面进行分析比较。
High performance liquid chromatography（HPLC）is one of the most important factors in monosaccharide analysis due to its simple operation,and high efficiency and sensitivity.In this study,several commonly used methods were described,for the analysis of monosaccharides by high performance liquid chromatography,i.e.,liquid chromatography differential refractive index detection,liquid chromatography evaporative light scattering detection,PMP pre-column derivatization detection,para-aminobenzoic acid pre-column derivatization,the o-aminobenzoic acid derivative method and post-column derivatization method.Moreover,the advantages and disadvantages of these methods were demonstrated,and the sensitivity,operation method,and accuracy were compared and analyzed among these methods.
作者
热孜万古力.赛买提
陈士恩
高丹丹
武中庸
车敏娜
李明生
SAIMAITI Reziwanguli,CHEN Shi-en,GAO Dan-dan,WU Zhong-yong,CHE Min-na,LI Ming-sheng(Northwest Minzu University, Lanzhou , Gansu 730030, Chin)
出处
《食品与机械》
CSCD
北大核心
2018年第2期185-190,共6页
Food and Machinery
基金
国家民委专项综合改革试点(编号:10019141)
国家科技支撑计划项目(编号:2015BAD29B05)
西北民族大学"双一流引导专项"(编号:10018704)
西北民族大学中央高校基本科研业务费专项资金(编号:31920170178)
甘肃省兰州市城关区科技计划项目(编号:2017KJGG0024)
甘肃省科技计划资助(编号:17YF1WA166)
关键词
高效液相色谱法
柱前衍生
柱后衍生
单糖
High performance liquid chromatography（HPLC）
precolumn derivation
post column derivation
monosaccharide
