五大连池药泉山矿泉微生物多样性及其地质和环境控制特征

Microbial Diversity of Mineral Spring and Its Geological and Environmental Controls in Yaoquan Mountain,Wuda-Lianchi of NE China

药泉山矿泉是五大连池世界地质公园旅游疗养业赖以生存和发展的重要资源,然而其矿泉水的微生物研究一直是空白。本文在对五大连池药泉山矿泉环境水文地质前期成果的基础上,选择典型季节对该区域的矿泉水进行现场采样和分析,并进一步利用分子生物学PCR-DGGE技术对水样的微生物群落多样性进行了初步的研究与分析。结果表明:研究区的微生物多样性显著,而且不同季节微生物多样性统计参数存在巨大差异;在不同的空间位置夏季微生物多样性变化较大,但是同一位置的总体变化规律基本一致,这说明矿泉水中的微生物对地质特征、环境条件的响应具有良好的一致性;而冬季的微生物多样性统计参数在不同空间位置的变化规律也呈现明显差异,但是其他参数变化不大,对地质空间、环境条件响应微弱。该研究对揭示五大连池矿泉水微观成因具有一定的价值。

The mineral springs in the Yaoquan Mountain have been the important resource for the tourism and therapy in the Wuda-Lianchi geological resort,and attracted much more research in recent years,but no one is for the microbial diversity of the mineral spring.Based on the previous environmental hydrogeology of this area,the sampling and analysis of the springs in the typical seasons were undertaken,and further the molecular biology work for the microbial diversity was conducted by the PCR-DGGE technique.The results showed that there was a distinct micro-diversity in the study area,and the micro-statistical parameters were greatly different in different seasons.The micro-diversity changed a lot at various spatial locations in summer,but generally consistent at the same location,showing that microorganisms of the springs responded well to the geological settings and environmental conditions.There were clear differences for the statistical parameter of microbes at different spatial locations in winter,but the other parameters changed little.This study is scientifically valuable for the insight of the springs formation at a micro-level in Wuda-Lianchi.