青藏高原垂穗披碱草种质资源遗传多样性的SSR分析

Genetic diversity of Elymus nutans germplasm resources from the Qinghai-Tibet Plateau in China detected by SSR markers

采用SSR分子标记技术,对采自青藏高原的30份野生垂穗披碱草(Elymus nutans)种质进行遗传多样性分析。结果表明,1)筛选出的16对引物共获得了116个等位基因位点,92个多态性位点,多态性位点率(PPB)为79.75%,多态性信息(PIC)含量为0.063~0.325,平均值为0.188,材料间遗传相似系数(GS)介于0.692~0.976,平均值为0.828。2)聚类分析表明,在遗传相似系数0.804处,30份供试材料可聚为四大类,单独聚为一类的09-214表现出与其他材料较远的亲缘关系,主成分分析结果与UPGMA结果基本一致。群体遗传结构分析显示,大部分材料的遗传背景较为单一,群体划分的结果与聚类分析的结果部分保持一致。研究显示,供试种质材料间存在较大的差异,部分材料表现出相对独立的特性,遗传多样性较丰富,可为垂穗披碱草的保护利用、新品种的选育及优良基因的挖掘提供参考依据。

SSR markers were used to analyze the genetic diversity of 30 accessions of wild Elymus nutans collected from the Qinghai-Tibet Plateau.The results showed that：1）A total of 116 fragments and 92 polymorphic loci were generated from 16 pairs of primers.The polymorphic loci rate（PPB）was 79.75%,with polymorphism information contents（PIC）ranging from 0.063 to 0.325（mean 0.188）.The genetic similarity（GS）coefficient ranged from 0.692 to 0.976（mean 0.828）.2）A cluster analysis showed that at the genetic similarity coefficient of 0.804,the 30 samples clustered in four major groups.Individually clustered groups of 09-214 showed distant genetic relationships with other accessions.The result of a principal component analysis was generally consistent with those of a UPGMA.The analysis of population genetic structure indicated that most of the samples may originate from the same genetic background,and results of grouping analysis were consistent with those of the cluster analysis.Our results showed a great difference between the tested germplasm samples,with several of the accessions showing relatively independent characteristics and higher genetic diversity,which could provide reference for the protection and utilization of E.nutans,the selection of new varieties and the discovery of＂good genes＂.