结缕草新品种DUS测试数量性状特异性判别

Study of the assessment of quantitative characteristics in DUS testing of new zoysiagrass varieties

DUS测试是植物新品种保护的技术基础和授权依据。本研究按照农业部发布的结缕草属(Zoysia)DUS测试指南,对包括两个申请品种及其近似品种和标准品种在内的10份结缕草材料进行了连续两个独立生长周期的DUS测试,并使用代码法和数理统计分析法两种方法对8个数量性状的特异性进行判定,旨在为结缕草申请品种DUS数量性状的特异性判定提供依据。两种方法判定结果比较发现,使用代码法判定有特异性的数量性状不一定在统计学上有显著差异,而判定为没有特异性的数量性状在统计学上却可能有显著差异。为了避免特异性误判,对代码法判定有特异性的数量性状进一步做了数理统计分析。结果表明,广绿结缕草在匍匐茎节间长、叶长和种子千粒重3个数量性状上具备特异性;Z0413-1结缕草在匍匐茎节间长、叶宽和花序小穗数3个数量性状上具备特异性。

DUS testing provides a technical and authoritative basis for the protection of new plant varieties.Ten zoysiagrass varieties,including two candidate varieties,two similar varieties,and standard varieties,were selectedin the present study for DUS testing over two consecutive growing seasons.The test was strictly guided by DUS testing guidelines,published by the Ministry of Agriculture of China.The distinctness of eight quantitative characteristics were assessed by two methods（note and statistical analysis）to provide practical evidence for the assessment of quantitative characteristics of zoysiagrass DUS testing.The assessment of results from the two methods showed that quantitative characteristics with distinctness that were assessed by the notemethod showed non-significant differences by the statistical analysis method,whereas characteristics without distinctness showed significant differences by the statistical analysis method.To avoid such misjudgment of distinctness,the quantitative characteristics with distinctness assessed by the notemethod were further analyzed with the statistical analysis method.The results showed that the candidate variety of zoysiagrass Guanglühad distinctness in quantitative characteristics of stolon length of internode,leaf length,and seed kilo grain weight,whereas the candidate variety of zoysiagrass Z0413-1 had distinctness in quantitative characteristics of stolon length of internode,leaf width,and inflorescence numbers of spikelet.