白芨生肌膏对肛肠病术后创面HSP70基因表达的临床观察

Clinical observation on wound HSP70 gene expression after anorectal disease of Baiji Shengji Ointment

目的观察白芨生肌膏在肛肠术后患者创面愈合中对热休克蛋白70(HSP70)表达的影响,从分子生物学层面评价白芨生肌膏对肛肠疾病手术后创面愈合的临床疗效,提供该治疗方法的客观依据。方法选择符合诊断标准的肛肠疾病并进行手术治疗的患者40例,随机分为治疗组(n=20)和对照组(n=20)。治疗组给予本科白芨生肌膏,排便后换药,覆盖在创面;对照组给予马应龙龙珠软膏纱条,方法同前。分别取两组患者术后第1、3、7天创面组织检测HSP70的表达量。结果术后第1天,治疗组与对照组创面组织中HSP70表达量比较差异无统计学意义;术后第3、7天,治疗组创面组织中HSP表达量有差异明显高于对照组(P<0.05)。结论白芨生肌膏能促进肛肠病术后创面的愈合,提高创面组织对HSP70表达。同时该方法安全有效、制作简单、容易操作、药简价廉,在临床中能更好地推广。

Objective To observe the effect of Baiji Shengji ointment on heat shock protein 70 in patients with wound healing after anorectal operation in（HSP70） expression, from molecular biology to evaluate the level of Baiji Shengji ointment on the clinical efficacy of wound healing after anorectal disease surgery, provide the treatment objective basis. Methods 40 cases of anorectal diseases and surgical treatment were selected and randomly divided into the treatment group（n=20） and the control group（n=20）. The treatment group received undergraduate Baiji Shengji ointment,defecation after dressing, covering the wound; the control group was given Mayinglong Longzhu ointment gauze, with the former method. The expression of HSP70 in the wound tissue was detected at first, third, seventh days after operation in two groups. Results On the first day after operation, there was no significant difference in the expression of HSP70 between the treatment group and the control group. The expression of HSP in the wound tissue of the treatment group was significantly different from that of the control group on the third, seventh day after operation（P〈0.05）.Conclusion Baiji Shengji ointment can promote the healing of wound after operation for anorectal diseases, improve the expression of HSP70 in wound tissue. At the same time, the method is safe and effective, easy to make, easy to operate, low cost, and can be better popularized in clinical.