不同针刺法对C57/BL6小鼠黑素生成、ACTH及相关细胞因子表达的影响

The effect of different acupuncture methods on C57/BL6 mice pigmentogenesis,ACTH and related cytokines expression

目的:观察不同针刺法对C57/BL6小鼠黑色素生成、促肾上腺皮质激素(ACTH)及相关细胞因子(IL-1β、TGF-β、TNF-α)表达的影响及作用机制。方法:将48只小鼠随机分为空白组、火针组、普通针刺组及冰针组,肉眼观察小鼠皮肤黑色素生成情况,并行组织学检查,采用免疫组化法检测小鼠皮肤中ACTH、IL-1β、TGF-β、TNF-α的表达。结果:火针组最早出现黑色素生成,至第14天,HE染色中黑色素颗粒明显多于普通针刺组及冰针组。至第3天,各针刺组ACTH表达均高于空白组(t值分别为9.36、6.37、5.87,P值均<0.05),其中火针组表达强于普通针刺组及冰针组(t值分别为3.42、2.93,P值均<0.05)。与之对应,IL-1β在火针组的表达均强于普通针刺组、冰针组,第3天最明显(t值分别为2.88、2.94,P值均<0.05);TGF-β第3天可见火针组表达强于普通针刺组、冰针组,差异有统计学意义(t值分别为3.01、3.45,P值均<0.05)。各组间TNF-α的表达则无明显改变。结论:不同针刺法均明显促进C57/BL6小鼠黑色素生成,上调ACTH、IL-1β及TGF-β的表达,其中以火针组作用最为明显。

Objective： To analyze the function of different accupucture in C57/BL6 mice pigmentogenesis,and detect the expression of ACTH and related cytokines,including IL-1β、TGF-β、TNF-α during the process. Methods： Forty-eight C57/BL6 mice were used as the experiment subjects,certain area of each mouse skin was irradiated with fire needles,ice needles or normal acupuncture respectively. Efficacy was evaluated at the 1 st day,3 rd day,7 th day and 14 th day of the experiment by observation,HE staining,immunohistochemistry to stain ACTH、IL-1β、TGF-β and TNF-α. Results： The expression of ACTH could be detected in the beginning in every needle group,which was obviously higher than that of blank group（ t = 9. 36,6. 37,5. 87,P〈0. 05） at the 3 rd day,and the expression of ACTH in fire needle group were higher than common-needle group and ice-needle group（ t = 3. 42,2. 93,P〈0. 05）. After the fire-needle treated,the expression of IL-1β on the 3 rd day was stronger than common-needle group or ice-needle group（ t =2. 88,2. 94,P〈0. 05）. The expression of TGF-β is stronger than common-needle group,ice-needle group on the 3 rd day（ t = 3. 01,3. 45,P〈0. 05）. However,the expression of TNF-α in different periods of different groups had no obvious differences. Conclusion： Different acupuncture methods could increase the expression of ACTH,enhance melanogenesis. The effect of the fire needle group is the most obvious.