乏情和发情初产母猪卵巢microRNA差异表达分析

Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows

为了探索卵巢microRNA(miRNA)在初产母猪生殖调控中的作用,本研究利用Illumina高通量测序技术检测了乏情和发情初产母猪卵巢miRNA的表达谱,并对表达量较高且差异显著的miRNA进行生物信息学分析。结果显示,本研究构建的两个small RNA文库共鉴定出503个miRNAs,其中已知的303个,新预测的200个。在已知的miRNA中,ssc-miR-10b在两个文库中的表达量最高,其次为ssc-miR-143-3p和ssc-miR-26a;在新预测的miRNA中,chr13_2637_mature在两个文库中的表达量最高,其次为chr8_9994_mature。与发情母猪相比,共有145个miRNAs发生显著变化(read counts>10,∣log2(fold-change)∣>1),上调114个,下调31个。在进一步筛选的31个表达量较高且差异显著的miRNAs(read counts>1 000,∣log2(fold-change)∣>1)中,新预测的chr13_2585_mature上调倍数最高,且只在乏情母猪卵巢中表达。31个miRNAs共预测到7 388个靶基因,KEGG信号通路分析显示,有2 788个靶基因注释到了297个KEGG通路,前20个最富集的通路部分与生殖过程或生殖活动调控相关,表明这31个miRNAs参与了初产母猪的生殖调控。本研究结果丰富了猪miRNA数据资源,为进一步深入研究初产母猪的繁殖性能提供了理论依据。

To explore the role of ovarian microRNA （miRNA） in regulating the reproductive process in primiparous sows,the miRNA expression profiles were examined using Illumina high-throughout sequencing.The miRNA with high expression and significant difference were analyzed by bioinformatics methods.The results showed that a total of 503 miRNAs were detected in two small RNA libraries,which included 303 known and 200 novel miRNAs.The 3 most highly expressed miRNAs in known miRNAs were ssc-miR-10b,ssc-miR-143-3p and ssc-miR-26a in two small RNA libraries.The highest specific expressed miRNAs in novel miRNAs was chr132637mature,followed by chr89994mature.Compared with estrous primiparous sows,a total of 145 differentially expressed miRNAs （read counts〉10, ｜log 2（fold-change）｜〉1）,including 114 upregulated miRNAs and 31 downregulated miRNAs were identified.31 differentially expressed miRNAs （read counts〉1 000,∣log 2（fold-change）∣〉1） were further screened.In these miRNAs,chr132585mature was the highest-upregulated,and was discovered only in the ovary of anestrous primiparous sows.Furthermore,7 388 target genes were predicted from 31 miRNAs.KEGG pathway analyses showed that a total of 2 788 target genes were annotated to 297 pathways,and the top 20 most significantly enriched pathways were involved in reproductive processes,suggesting the 31 miRNAs played a crucial role in the reproductive process of primiparous sows.These results enriched the porcine miRNA database and provided useful information for further research about the reproductive performance of primiparous sows.