新疆北疆地区猪源粪肠球菌的耐药性分析

Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang

为了解新疆北疆地区猪源粪肠球菌的耐药性及相关耐药基因型的分布情况,本试验采用K-B(Kirby-Baller)琼脂扩散法检测了49株猪源粪肠球菌对8种抗菌药物的敏感性,并采用PCR法对9种相关耐药基因进行检测并测序,测序结果与GenBank中的相应基因序列比对。药敏试验结果显示,分离菌对链霉素耐药率最高,其次为青霉素和红霉素,对呋喃妥因、氨苄西林高度敏感。PCR检测结果显示,β-内酰胺类耐药基因tem的检出率最高,为93.88%,其次是四环素类耐药基因tetM,为85.71%,喹诺酮类基因gyrA和parC检出率均为42.86%,氨基糖苷类耐药基因aph(3′)-Ⅲ、aac(6′)/aph2″和ant(6′)-Ⅰ的检出率分别为36.73%、16.33%和16.33%,未检出mefA和ermB基因。本试验从表型与基因型分析发现,北疆地区猪源粪肠球菌的多重耐药现象非常严重,且其耐药表型与基因型并不完全一致。

In order to determine the drug resistance of swine Enterococcus faecalis and the distribution of genotypes resistance in Northern Xinjiang,total 49 isolates were evaluated for their sensitivity to 8 antimicrobial drugs by Kirby-Baller disc diffusion method,and 9 relative resistance genes were detected by PCR method.The amplified fragments were sequenced and compared with the corresponding gene sequences in GenBank.Susceptibility test results showed that the isolates had the highest resistance rate to streptomycin,followed by penicillin and erythromycin,which were highly sensitive to nitrofurantoin and ampicillin.PCR detection results showed that the detection rate ofβ-lactam resistance gene tem was the highest（93.88%）,followed by the tetracycline resistance gene tetM（85.71%）,the detection rates of quinolones resistance gene gyrAandparCboth were 42.86%,the detection rates of aminoglycoside resistance genes aph（3′）-Ⅲ,aac（6′）/aph2″and ant（6′）-Ⅰ were 36.73%,16.33% and 16.33%,respectively,and no mefA and ermBgenes were detected.The results showed that the multidrug resistance of swine Enterococcus faecalis in Northern Xinjiang was very serious,and its resistance phenotype was not consistent with the genotype.