牙髓干细胞在组织工程研究中的进展和应用前景

Dental pulp stem cells in tissue engineering: application and development

背景:牙髓干细胞作为特殊的干细胞来源,具有高度增殖和自我更新能力,能在特定条件诱导下分化成多种组织细胞,为组织工程领域的发展寻觅了一条新的途径。目的:对牙髓干细胞的生物学特征、来源、分离方法及其在组织工程学中的应用和研究等方面作一综述。方法:由第一作者以"dental pulp stem cell,differentiation,regenerative medicine,tissue engineering"为英文关键词,以"牙髓干细胞,分化,再生医学,组织工程"为中文关键词,检索2005至2017年期间收录在Pub Med,Medline,中国知网,万方等数据库中与牙髓干细胞和组织工程医学相关的文献,排除与文章研究目的无关及重复性的文献,纳入符合标准的66篇文献进行综述。结果与结论:随着组织工程医学和再生医学应用的不断发展,牙髓干细胞和组织工程支架的有效结合得以实现,目前研究的重点不仅仅关注于牙髓干细胞的成牙、成骨特性,其在神经修复、血管构建、角膜重建、软骨形成等方面也得到广泛应用。

BACKGROUND： As a special source of stem cells, dental pulp stem cells （DPSCs） make much progress in the development of tissue engineering field due to their high proliferation and self-renewal ability. In the certain conditions DPSCs can be induced to differentiate into a variety of specialized tissue cells, providing a new way for tissue engineering development. OBJECTIVE： To review the main progress in the DPSCs biological characteristics, original source, isolation method, and its related application in tissue engineering research. METHODS： ＂Dental pulp stem cell, differentiation, regenerative medicine, tissue engineering＂ in English and Chinese were termed as the keywords to search relevant articles about DPSCs and tissue engineering published from 2005 to 2017 in PubMed, Medline, WanFang, and CNKI databases. After removal of repetitive or irrelevant articles, 66 articles were finally reviewed. RESULTS AND CONCLUSION： With the development of tissue engineering and regenerative medicine, the effective combination of DPSCs and tissue engineering scaffolds have be further achieved. Recent studies on DPSCs focus on the properties of DPSCs differentiating into odontoblasts and osteocytes/osteoblasts and on the potential of nerve repair, vascular remodeling, corneal reconstruction and chondrogenic differentiation.