摘要
以衡水湖外来入侵植物黄顶菊Flaveria bidentis为材料,对其不同部位内生细菌分离、纯化,用分离到的内生菌与苹果轮纹病菌Botryosphaeria dothidea进行对峙培养。结果表明:从黄顶菊根、茎、叶、花中分别分离到4,4,4,2株共计14株内生细菌;对峙培养筛选出5株(茎1、茎2、茎4、花1、花2)对苹果轮纹病菌抑制作用明显的细菌,5菌株之间的抑菌差异性不显著;菌株茎4抑制效果最好,真菌菌落直径为(39.96±2.66)mm,抑菌率为33.21%。
Endophytic bacteria were isolated from Flaveria bidentis, and was antagonistic screening with Botryosphaeria dothidea. The results showed that： 14 strains were isolated from roots, stems, leaves and flowers of F. bidentis. Five （ stem 1, stem 2, stem 4, flower 1, flower 2 ） strains were screened through the antagonism experiment between endophytic bacteria and B. dothidea. There was no signifi- cant difference in bacteriostatic effect among the five strains. Stem 4 had the most significant effect with bacteriostasis rate being 33.21% and colong diameter being （39.96 ＋2.66） mm.
作者
侯晓杰
梁魁景
欧阳汝欣
芦站根
HOU Xiaojie(Department of Life Science, Hengshui University, Hengshui 053000, Chin)
出处
《中国森林病虫》
2018年第3期38-40,共3页
Forest Pest and Disease
基金
河北省高等学校青年拔尖人才计划资助项目(BJ201406)
衡水市科学技术研究与发展计划资助项目(14064)
河北省科技计划项目(15226505)
关键词
苹果轮纹病菌
黄顶菊
内生菌
拮抗
Botryosphaeria dothidea
Flaveria bidentis
endophytic bacteria
antagonistic
