KCNQ1基因7个位点单核苷酸多态性与糖尿病前期人群运动干预敏感性的关联

Relationship between Single Nucleotide Polymorphisms in Seven Sites of KCNQ1 and Susceptibilities to Exercise in Prediabetes

目的探讨钾离子通道蛋白KCNQ1基因7个标签位点单核苷酸多态性与糖尿病前期人群运动干预效果的关系。方法 2015年1月,对70例糖代谢异常者进行有氧耐力训练12周,测定干预前后糖脂代谢相关生化指标,采用基质辅助激光解吸电离飞行时间质谱法解析受试者基因组基因型。结果最终66例完成检测。与rs2299620位点其他基因型相比,TT基因型餐后2 h血糖最低(F=5.460,P<0.05);与其他单体型相比,rs2237897-rs2299620位点C-T单体型、rs2299620-rs151290位点T-C单体型、rs2299620-rs2237892位点T-T单体型餐后2 h血糖下降更多(χ~2>7.950,P<0.05),rs2237897-rs2299620位点T-C单体型空腹胰岛素下降更多(χ~2=9.000,P<0.05),rs2283228-rs2237892位点A-C单体型低密度脂蛋白下降更多(χ~2=15.820,P<0.001)。结论 KCNQ1基因rs2299620位点的TT基因型以及多个单体型人群,糖脂代谢对运动相对敏感。

Objective To observe the relationship between 7 single nucleotide polymorphisms（SNPs） in KCNQ1 gene region and the effects of exercise intervention on prediatbets.Methods From January, 2015, 70 prediabetes accepted aerobic endurance exercise for twelve weeks. The indexes related to glucose and lipid metabolism were measured before and after intervention. Their genotypes of SNPs were detected with matrix-assisted laser desorption/ionization time of flight mass spectrometry.Results A total of 66 cases finished the tests. Compared with other genotypes of rs2299620, two hours postprandial blood glucose（P2 h BG） decreased the most in those with TT genotype（F=5.460, P〈0.05）. Compared with other haplotypes, P2 h BG decreased more in those with C-T haplotype of rs2237897-rs2299620, T-C haplotype ofrs2299620-rs151290 and T-T haplotype of rs2299620-rs2237892（χ~27.950, P〈0.05）, fasting insulin decreased more in those with T-C haplotype of rs2237897-rs2299620（χ^2=9.000, P〈0.05）, and low density lipoprotein decreased more in those with A-C haplotype of rs2283228-rs2237892（χ^2=15.820, P〈0.001）.Conclusion The prediatbets with TT genotype of rs2299620 and some haplotypes of KCNQ1 are susceptible to exercise intervention in glucose and lipid metabolism.