黄芩苷对海人酸致癫小鼠Toll样受体2／4信号通路的影响

Effects of baicalin on the expression of Toll - like receptor 2/4 signaling pathway in kainate - induced epileptic mice

目的 观察黄芩苷对小鼠癫痫持续状态后Toll样受体2（TLR2）、Toll样受体4（TLR4）信号通路的影响,探讨黄芩苷对癫痫发作后神经细胞保护作用的机制.方法 48只ICR雄性小鼠随机分为对照组、癫痫持续状态组、黄芩苷治疗组（100 mg/kg）.采用腹腔内注入海人酸（12 mg/kg）建立小鼠癫痫持续状态模型.通过苏木素-伊红（HE）染色、尼氏染色观察小鼠海马组织神经细胞的形态学变化及坏死情况,免疫组织化学观察海马组织中TLR2、TLR4的细胞阳性表达,采用反转录-聚合酶链反应（RT-PCR）、Western blot检测TLR2、TLR4、核转录因子-κB （NF-κB） mRNA和蛋白的表达量.结果 黄芩苷治疗组可显著减轻癫痫所致的海马神经细胞的损伤,降低神经细胞坏死（114.27±15.53）,同时下调海马组织中TLR2、TLR4、NF-κB mRNA和蛋白的表达（P=0.043）.结论 癫痫发作后可激活TLR2、TLR4介导的炎症信号通路;黄芩苷可通过抑制TLR2、TLR4介导的炎症信号通路,降低癫痫发作后海马神经细胞的损伤.

Objective To study the effects of baicalin on the expression of Toll-like receptor 2/4 （TLR2/4） signaling pathway in Kainate-induced epileptic mice,and explore the neuroprotective mechanism of baicalin after status epilepticus （SE）.Methods Forty-eight ICR male mice were randomly divided into three groups：control group,SE group and baicalin group,to which baicalin was administered at doses of 100 mg/kg.To establish animal model of SE,12 mg/kg kainic acid was administered to mice by intraperitoneal injection.Hematoxylin and eosin （HE） stain was used to observe the pathological changes.Nissl staining was used to assess the necrosis of hippocampal neurons.Immunohistochemistry was used to observe the positive expression of TLR2 and TLR4.Reverse transcription polymerase chain reaction （RT-PCR） and Western blot was used to detect the expression of the mRNA and protein 1eveI of TLR2,TLR4 and nuclear factor-κB （NF-κB）.Results Baicalin can significantly relieve the injury and inhibit granule cells losts （114.27 ± 15.53）,and at the same time decreas the expression of TLR2,TLR4 and NF-κB （P =0.043）.Conclusion TLR2/4 signaling pathway could be activated after SE and Baicalin could inhibit TLR2/4-mediated signaling pathway to protect the hippocampal neurons after SE in mice.