乳腺癌扩增性抗原1在胆管细胞性肝癌组织的表达及其意义

Expression and significance of amplified in breast cancer 1 in intrahepatic cholangiocarcinoma

目的 探讨乳腺癌扩增性抗原1（AIB1）和上皮-间充质转化（EMT）相关标记分子在胆管细胞性肝癌（ICC）中的表达及其临床意义.方法 免疫组织化学法检测AIB1在癌旁组织及ICC组织中表达,分析AIB1与ICC临床病理的关系.结果 免疫组织化学检测结果显示,ICC组织中AIB1蛋白表达水平明显高于癌旁组织,与癌旁组织比较,ICC组织中N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）表达水平也明显升高.ICC组织中的AIB1蛋白表达水平与患者年龄、性别、肿瘤直径、远处转移及病理分型无相关性.AIB1阳性表达的发生淋巴结转移性ICC患者发生率是48.71％ （19/39）,而AIB1阴性表达的发生淋巴结转移性ICC患者发生率是24.32％ （9/37）,两者差异有统计学意义（χ2=4.855,P=0.035）.此外,N-cadherin阳性表达的发生淋巴结转移性ICC患者发生率是47.50％ （19/40）,而N-cadherin阴性表达的发生淋巴结转移性ICC患者发生率是25.00％（9/36）,两者差异有统计学意义（χ2=4.122,P=0.027）.Vimentin蛋白表达水平与患者年龄、性别、肿瘤直径、远处转移及病理分型无相关性.Western blot结果表明,淋巴结转移阳性胆管癌组织AIB1表达水平明显高于淋巴结转阴性胆管癌组织,差异有统计学意义（χ2=0.625,P=0.034）.结论 AIB1在ICC中表达增加,且与ICC淋巴结转移和EMT基因相关,提示AIB1可能通过诱导ICC、EMT,可促进ICC发生侵袭性转移.

Objective To investigate the amplified in breast cancer 1 （AIB1） and epithelial mesenchymal transition （EMT） related molecular markers in intrahepatic cholangiocarcinoma （ICC） and its relationship with clinical pathology.Methods The expression of AIB1 was detected by immunohistochemistry in the tissue adjacent to carcinoma and ICC.Recation between AIB1 clinical pathology of ICC was analyzed.Results The results of immunohistochemistry showed that the expression of AIB1 protein in ICC tissue was significantly higher than that in adjacent tissues,and the expression level of N-cadherin and Vimentin in ICC tissue was significantly higher than that in adjacent tissues.There was no significant difference in AIB1 protein expression with age,sex,tumor diameter,distant metastasis and pathological typing.The lymph node metastasis of ICC cases in positive expression of AIB1 occurred was 48.71% （19/39）,and lymph node metastasis of ICC in negative expression of AIB1 occurred was 24.32% （9/37）,there was statistically significant （P =0.035）.In addition,the lymph node metastasis of ICC cases in positive expression of N-cadherin occurred was 47.50% （19/40）,and lymph node metastasis of ICC in negative expression of N-cadherin occurred was 25.00% （9/36）,two with statistical difference （P =0.027）.There was no significant difference in Vimentin protein expression with age,sex,tumor diameter,distant metastasis and pathological typing.Western blotting assay results showed that AIBl expression in cancer tissue with positive lymph node metastasis was significantly higher than that in cancer tissue with negative lymph node metastasis,the difference was statistically significant （P =0.034）.Conclusion The expression of AIB1 in ICC is associated with ICC lymph node metastasis and epithelial mesenchymal transition,suggesting that AIB1 may promote the invasion and metastasis of ICC by inducing epithelial mesenchymal transition of ICC.