IL-8诱导卵巢癌细胞发生上皮-间质转化初探

IL-8Induces Epithelial-to-mesenchymal Transition of Ovarian Carcinoma Cells:a Preliminary Study

目的初步探讨白细胞介素-8(Interleukin-8,IL-8)在诱导卵巢癌细胞发生上皮-间质转化(EMT)中的作用,为进一步深入研究卵巢癌恶性转移的分子机理奠定基础。方法实时无标记细胞技术(RTCA)定量分析外源性人重组IL-8诱导卵巢癌细胞株SKOV3的动态迁移情况;Western blot检测IL-8(100ng/mL)诱导处理SKOV3细胞48h后EMT相关蛋白的表达变化情况;采用Transwell分析经IL-8诱导处理后的SKOV3细胞侵袭情况。结果 RTCA定量结果显示,IL-8处理SKOV3细胞48h后细胞的迁移达到平台期;IL-8可使SKOV3细胞的上皮细胞标志物E-cadherin下调,而间质细胞标志物Vimentin和snail的表达上调,促进卵巢癌细胞发生EMT,SKOV3细胞的侵袭能力明显增强(P<0.05)。结论 IL-8可促使卵巢癌细胞获得EMT特性,从而增强其侵袭、迁移能力。

Objective To explore the effect of IL-8 on the epithelial-to-mesenchymal transition（EMT）in ovarian cancer,which will provide experimental basis for revealing related molecular mechanism in malignant metastasis of ovarian cancer.Methods The migration of ovarian cancer cell line SKOV3 cells was explored with Real time label free cell analysis（RTCA）after treatment with recombinant human IL-8.SKOV3 cells were cocultured with IL-8 for 48 h,proteins involved in EMT were investigated via Western blot to explore the effect of IL-8 on the activation of the EMT.Invasion of SKOV3 cells after treatment with IL-8 were evaluated by transwell assay.Results According to the results of RTCA,after treatment with IL-8 for 48 h,the migration of SKOV3 cells was in platform phase.The treatment of IL-8 unregulated vimentin and snail and downregulated E-cadherin,which suggested that IL-8 induced EMT in ovarian cancer.The results of transwell test showed that invasive ability of IL-8 pretreated SKOV3 cells was enhanced（P〈0.05）.Conclusion IL-8 can induce the EMT of ovarian cancer and enhance the invasion and migration of ovarian cancer.