解偶联蛋白2基因-866G/A,Ala55Val及Ins/Del多态性与宁夏回族人群脑缺血发病风险的相关性研究

Associationstudy between single nucleotide polymorphism of UCP2-866G/A,Ala55Val,Ins/Del and cerebral ischemia stroke attack in ningxia hui population

目的探讨UCP2基因多态性在回族人群中的分布特点及UCP2SNP与脑缺血发病风险的相关性。方法收集宁夏地区回族正常人群及回族脑缺血患者外周血样本,提取外周血基因组DNA,采用聚合酶链反应-限制性片断长度多态性方法检测各样本UCP2基因-866G/A,Ala55Val及Ins/Del多态性。结果UCP2基因-866G/A,Ala55Val及Ins/Del多态性在宁夏地区回族正常人群与脑缺血患者中分布频率无明显差异(P>0.05)。结论 UCP2多态性与宁夏地区回族脑缺血发病无相关性。

Objective To explore the features of UCP2 gene polymorphism distribution of people from Hui minority in Ningxia,and the association between single nucleotide polymorphism of Uncouple protein 2（ UCP2）-866 G/A,Ala55 Val,Ins/Del and cerebral ischemia stroke attack. Methods After extracted genomic DNA from the collected peripheral blood in patients with cerebral ischemia and the normal,the PCR-RELF method was utilized to test the single nucleotide polymorphism of UCP2-866 G/A,Ala55 Val,Ins/Del. Results There was no statistical difference about the frequencies of single nucleotide polymorphism of UCP2-866 G/A,Ala55 Val,Ins/Del between the normal control group and the stroke group in Ningxia Hui population. Conclusion This study reveals that there is no relationship between single nucleotide polymorphism of UCP2-866 G/A,Ala55 Val,Ins/Del and cerebral ischemia stroke attack in Ningxia Hui population.