丙型肝炎病毒抗体检测中酶联免疫和胶体金法的应用效果

Application effect of enzyme-linked immunoassay and colloidal gold method in antibody detection of hepatitis C virus

目的研究丙型肝炎病毒抗体检测中酶联免疫和胶体金法的应用效果。方法选取我院2015年3月至2017年3月收治的980例疑似丙型肝炎患者作为研究对象,所有患者均选用酶联免疫法与胶体金法进行检测。比较酶联免疫法与胶体金法的检测时间、检测费用及阳性检测率。结果与酶联免疫法比较,胶体金法的检测时间更短,检测费用更低(P<0.05);与胶体金法比较,酶联免疫法的阳性检出率略高,但两种检测方式的阳性检出率比较,差异无统计学意义(P>0.05)。结论丙型肝炎病毒抗体检测中酶联免疫和胶体金法的应用各有优缺点,胶体金法的检测时间较酶联免疫法更短、费用少于酶联免疫法,但酶联免疫法的阳性检测率略高于胶体金法。临床上丙型肝炎病毒抗体检测中应根据患者具体情况选用相应检测方式。

Objective To study the effect of enzyme-linked immunoassay and collo idal gold method in antibody detection of hepatitis C virus. Methods A total of 980 patients suspected with hepatitis C antibody admitted in our hospital from March 2015 to March 2017 were selected as the study objects, all the patients were tested by enzyme-linked immunoassay and colloidal gold method. The detection time, detection costs and positive detection rates were compared between enzyme linked immunoassay and colloidal gold method. Results Compared with the enzyme-linked immunoassay,the detection time of the colloidal gold method was shorter, and the detection cost was lower（P〈0.05）. Compared with the colloidal gold method, the positive detection rate of enzyme-linked immunoassay was higher, but there was no significant difference in the positive detection rates between the two detection methods（P〉0.05）. Conclusion Hepatitis C virus antibody detection of enzyme-linked immune and colloidal gold method have their advantages and disadvantages respectively. Compared with the colloidal gold method, enzyme-linked immunoassay method has shorter detection time and less detection cost, but enzyme-linked immunoassay positive detection rate is slightly higher than that of colloidal gold method. Clinical detection of hepatitis C virus antibodies should be based on the specific conditions of patients with the selection of appropriate detection methods.