外用前列腺素联合窄谱中波紫外线对白癜风豚鼠模型黑色素及酪氨酸酶水平的影响

Influence of combination of external prostaglandin and narrow band ultraviolet B on skin melanin and tyrosinase level in Guinea pigs with vitiligo

目的研究外用前列腺素联合窄谱中波紫外线(NB-UVB)对白癜风豚鼠的黑色素及酪氨酸酶水平的影响。方法以外涂3%氢醌霜制作白癜风豚鼠模型。按照体重将豚鼠随机分成6组,每组6只:正常组、模型组、对照组、单用药物组、单用光照组和联合组。正常组与模型组不予任何处理。对照组,外用等量凡士林,2次/天;单用药物组,地诺前列酮酊0.25 mg·m L^(-1),2次/天,连用2个月;单用光照组,将UV紫外线治疗仪置于距白斑部皮肤约20 cm处照射1 J·cm^(-1),波长为310~315 nm,每2 d进行1次,渐加量,连续照射2个月;联合组,照射、给药频次、方法及时间与单用药物组和单用光照组相同。治疗结束,在光镜下观察白癜风豚鼠的皮肤组织形态和黑色素分布情况。结果皮肤含黑色素毛囊百分率:模型组、对照组、单用药物组、单用光照组和联合组分别为(37.50±20.43)%,(53.33±14.38)%,(55.00±16.12)%,(46.67±19.66)%,(71.67±7.53)%,联合组与模型组相比,差异有统计学意义(P<0.05);但是,对照组、单用药物组和单用光照组与模型组相比,差异均无统计学意义(均P>0.05)。结论联合外用前列腺素与NB-UVB治疗白癜风有一定疗效。

Objective To investigate the influence of combination of external prostaglandin and narrow band ultraviolet B（ NB-UVB） on skin melanin and tyrosinase level in models with vitiligo. Methods Guinea pigs models with vitiligo were produced by 3% hydroquinone cream. And then all the animal models were randomly divided into six groups according to the weight： normal group,model group,control group,medication alone group,light alone group and combination group. Normal group and model group were not treated. Control group was used the same amount of vaseline,2 times per day; the 0. 25 mg · m L^-1 dipropionazone tincture skin smear in medication alone group,2 times per day,stay on 2 months;the NB-UVB irradiation treatment instrument was exposed to 1 J·cm^-1 at about 20 cm from the skin of the white spot,and the wavelength was310 ~ 315 nm in light alone group,and once every other day,stay on 2 months; combination group,topical prostaglandina combination NB-UVB irradiation,frequency,method and time same to medication alone group and light alone group. After administration,the observation of vitiligo guinea pig skin tissue morphology and melanin distribution in the lightmicroscope. Results The percentage of melanin hair follicles in model group,control group,medication alone group,light alone group and combination group were（ 37. 50 ± 20. 43） %,（ 53. 33 ± 14. 38） %,（ 55. 00 ± 16. 12） %,（ 46. 67 ± 19. 66） %,（ 71. 67 ± 7. 53） %,respectively. The combined group is compared to the model group,the difference is significant in statistics（ P〈0. 05）. But comparison between control group,medication alone group,light alone group and model group,the difference were not significant in statistics（ all P〉0. 05）. Conclusion Combination of external prostaglandins and NB-UVB have some effect on vitiligo treatment.