岷江百合与兰州百合鳞茎中多酚组分及其生物活性

Polyphenolic compositions and their bioactivity in the bulb of Lilium regale and L.davidii var.unicolor

为研究百合的抗氧化和抑菌活性,以野生岷江百合和兰州百合为材料,采用比色法、高效液相色谱法(HPLC)和比浊法对其鳞茎中总多酚、黄酮、黄烷醇、单体酚组分及含量、抗氧化能力和抑菌活性进行分析。结果表明:两种百合鳞茎中均富含多酚但种间存在显著差异(p<0.05),岷江百合鳞茎中总多酚、黄酮和黄烷醇的含量分别是兰州百合含量的2.07、2.25、3.16倍;在两种百合鳞茎中检测出14种单体酚,其中原矢车菊素B2含量最高,在岷江百合和兰州百合中分别达到了37.60、20.60 mg·kg^(-1),除没食子酸外,岷江百合鳞茎中其他被检测出的单体酚含量是兰州百合的1.43~41.84倍;两种百合鳞茎多酚提取物均具有一定的抗氧化和抑菌活性,且岷江百合的抗氧化和抑菌活性均显著高于兰州百合(p<0.05),是兰州百合的1.40~14.40倍。野生岷江百合鳞茎多酚提取物具有抗氧化和抑菌双重作用,可作为天然抗氧化剂、抗菌药物应用于食品和医药业,具有良好的开发应用前景。

To study the antioxidant and antibacterial activity of lily,using bulbs of Lilium regale and L.davidii var.unicolor as the experimental materials to investigate the contents of total polyphenol,flavone,flavanol and monomeric phenol of the bulb via colorimetry,high performance liquid chromatography and turbidimetry,and the antioxidant capacity and the antibacterial activity of that had been also stuied.The results demonstrated that there had a significant difference between the polyphenol contents in the bulbs of two Lilium species（p〈0.05）.The contents of total polyphenol,flavonoid,flavanol from L.regale were2.07,2.25 and 3.16 times higher than those of L.davidii var.unicolor,respectively.Fourteen monomeric phenolic compounds were identified from the two Lilium species,among them,the content of procyanidin B2 was the highest,reaching 37.60 mg·kg^（-1）and20.60 mg·kg^（-1）in L.regale and L.davidii var.unicolor respectively,and the contents of which in the bulb of Lilium regale were1.43 to 41.84 folds of those in L.davidii var.unicolor,except for the gallic acid content.Furthermore,the antioxidation and antibacterial activities of polyphenol extracts from Lilium regale were significantly 1.40-14.40 folds stronger than those from L.davidii var.unicolor（p〈0.05）.These findings suggested that polyphenolic extract of Lilium regale would possesse a great potential application prospect in the antioxidative food development and bacteriostatic pharmaceutical industry.