响应面法优化乳酸片球菌冻干保护剂配方

Optimization of cryoprotectants formulation for Pediococcus acidilactici by response surface methodology

添加冻干保护剂能有效提高微生物冻干存活率。为了提高乳酸片球菌冻干存活率,采用Plackett Burman实验设计与最陡爬坡实验结合响应面法,研究了不同冻干保护剂对冻干存活率的影响。结果表明,海藻糖、甘露醇、硫酸锰浓度对菌体存活率影响显著,海藻糖与甘露醇交互作用显著,海藻糖与硫酸锰,甘露醇与硫酸锰交互作用不显著。最佳保护剂配方:海藻糖浓度为73.64 g/L,甘露醇浓度为43.76 g/L,硫酸锰浓度为1.91 g/L,冻干存活率为98.10%±1.03%。实验结果为益生菌生产提供了技术支持。

The addition of freeze-drying cryoprotectants can improve the survival rate of microbial effectively. In order to increase the survival rate,cryoprotectants for Pediococus acidilactici were investigated using response surface methodology joined by Plackett Burman design and steepest ascend method. The results showed that trehalose,mannitol and manganese sulfate significantly affect the survival rate of Pediococus acidilactici. The interaction between trehalose and mannitol was remarkable,manganese sulfate did not interact with trehalose and mannitol remarkablely.The optimal amounts of cryoprotectants fomula were trehalose 73.64 g/L,mannitol 43.76 g/L,manganese sulfate 1.91 g/L and the survival rate reached 98.10% ±1.03%.The experimental results provided a technical support for the production of probiotics.