星点设计-效应面法优化枳雀内果皮柠檬苦素提取工艺

Optimization of extraction process of limonin from the Zhi Que'endocarp by central composite design-response surface methodology

采用表面活性剂异丙苯磺酸钠做水溶助剂替代有机溶剂,提取枳雀内果皮中的柠檬苦素,通过单因素实验考察水溶助剂浓度、水溶助剂用量、浸泡时间3个因素对枳雀内果皮柠檬苦素得率的影响,并采用星点设计-效应面法优化柠檬苦素提取工艺。结果表明:水溶助剂异丙苯磺酸钠用量、浓度、浸提时间的二次项对柠檬苦素得率影响极显著(p<0.0001),最佳工艺参数为异丙苯磺酸钠溶液用量27.7 m L/g,异丙苯磺酸钠溶液浓度2.3 mol/L,浸提时间7.0 h时,从枳雀内果皮提取到4.32 mg/g柠檬苦素,与响应面理论值4.37 mg/g接近,表明采用星点设计-效应面法优化枳雀内果皮柠檬苦素提取工艺的方法可行。

The surfactant sodium cumenesulfonate was used as the hydrotropic agent in place of organic solvent; the limonin in the endocarp of Zhi Que was extracted; three factors as the hydrotropic agent concentration,the hydrotropic agent dosage and the soak time were evaluated for the impact on the yield of the limonin in the endocarp of Zhi Que via single factor experiment;central composite design-response surface method was used to optimize the extraction process of limonin. The results showed that： the quadratic terms of the dosage,concentration and soak time of the hydrotropic agent sodium cumenesulfonate had significant effect on the yield of the limonin（ P＜0.0001）.When the optimum technology parameter was as follows： The dosage of sodium cumenesulfonate solution 27.7 m L/g,the concentration of sodium cumenesulfonate solution 2.3 mol/L,and the soak time 7.0 h,the limonin extracted from the endocarp of Zhi Que（ 4.32 mg/g） was close to the theoretical value on response surface 4.37 mg/g,indicating that the usage of central composite design-response surface method was feasible to optimize the extraction process of limonin.