RASSF2A基因甲基化与大肠癌的相关性分析

Analysis of correlation between RASSF2A gene methylation and colorectal cancer

目的 探讨大肠癌组织中RASSF2A基因启动子甲基化状态及其与大肠癌发生的相关性.方法 选择我院2007年2月到2013年3月收治的共111例散发性大肠癌患者,取其肿瘤组织及相关正常黏膜组织为标本,应用结合重亚硫酸盐限制性内切酶法（COBRA）检测RASSF2A基因在不同标本中的甲基化状态,分析RASSF2A基因甲基化与大肠癌临床病理特征的相关性.结果 RASSF2A基因甲基化在大肠癌组织及正常组织中分别为83.78％ （93/111）和7.21％ （8/111）,差异有统计学意义（x2=131.245,P＜0.001）.RASFF2A基因甲基化与大肠癌组织学分类（x2=3.554,P=0.314）、Duke分期（x2 =3.217,P =0.359）以及肿瘤部位（x2=1.060,P=0.303）无明显相关性.结论 大肠癌中存在RASSF2A的过甲基化,可能与大肠癌的发生有关.

Objective To investigate the methylation status of RASSF2A gene promoter in colorectal cancer tissues and its correlation with the occurrence of colorectal.Methods From February 2007 to March 2013,a total of 111 cases of sporadic colorectal cancer patients were selected.The methylation status of RASSF2A gene in tumor tissue and normal mucosa were detected by using combined bisulfite restriction endonuclease method （COBRA）.The correlations between the methylation of RASSF2A gene and the clinicopathologic features of colorectal cancer were analyzed.Results Methylation of RASSF2A gene was found in 83.78% （93/111） and 7.21% （8/111） of colorectal cancer tissues and normal tissues,with statistically significant difference （x2=131.245,P 〈 0.001）.The methylation of RASFF2A gene was not correlated with histology type （x2 =3.554,P =0.314）,Duke staging （x2 =3.217,P =0.359） and location of tumor （x2 =1.060,P =0.303）.Conclusion The presence of RASSF2A hypermethylation in colorectal cancer may be associated with the occurrence of colorectal cancer.