不同生境中桂花和夹竹桃叶际细菌的群落结构

Phyllosphere bacterial community structure of Osmanthus fragrans and Nerium indicum in different habitats

植物叶际微生物多样性是目前植物-微生物关系研究的热点之一,但影响叶际微生物群落结构的主要因素目前还存在很大争议.本研究以生长在3处生境的桂花和夹竹桃为对象,基于高通量测序技术,分析2种植物叶际细菌的群落结构,探讨影响植物叶际细菌群落结构的主要因素.结果表明:来自3处生境的2种植物叶际细菌多样性无显著差异,构成叶际细菌群落的优势门主要包括放线菌门、拟杆菌门、衣原体门、蓝细菌门、厚壁菌门和变形菌门,优势属主要包括甲基杆菌属、鞘氨醇单胞菌属、薄层杆属、Polaromonas和无毛螺旋体属.植物种类、生境及二者的交互作用均能显著影响叶际细菌群落结构,其中生境的影响最大.

The diversity of phyllosphere bacteria is one of the hotspots in the research area of plantmicrobial relationship. There are still a lot of controversies in the main factors influencing community structure of phyllosphere bacteria. In this study,the phyllosphere bacterial community structure of Osmanthus fragrans and Nerium indicum grown in three habitats was investigated based on highthroughput sequencing and the main driving factors were examined. The results showed that there was no significant difference in phyllosphere bacterial diversity between the two plant species grown in three habitats. For phyllosphere bacterial community of two plant species from three habitats,the dominant phyla were Actinobacteria,Bacteroidetes,Chlamydiae,Cyanobacteria,Firmicutes and Proteobacteria,and the dominant genera included Methylobacterium,Sphingomonas,Hymenobacter,Polaromonas and Spirosoma. The structure of phyllosphere bacterial community was influenced by habitats,host species identity and species-habitat interaction,among which habitat showed the strongest effect.