小鼠Willis环右颈总动脉灌注Microfil成像研究

The visualization of Willis circle by using Microfil perfusion through right common carotid artery in experimental mice

目的评估通过小鼠右侧颈总动脉途径灌注对比剂Microfil显示Willis环的可行性和效果。方法选取20只雄性C57BL/6小鼠(约14周龄,重约20 g)。将小鼠肝素化、麻醉后固定、解剖胸腔,依次自小鼠心尖灌注0.9%氯化钠溶液、4%多聚甲醛溶液和2%戊二醛溶液混合液;分离右侧颈总动脉,经右侧颈总动脉近端灌注Microfil,4℃过夜;取脑后体视镜下观察Willis环。将体视镜下观察到的Willis环定义为完全充盈、大部分充盈和充盈不良。结果 20只小鼠全部灌注成功,其中完全充盈11只(55%),大部分充盈4只(20%),充盈不良5只(25%)。大体观察小鼠Willis环完整,染色血管清晰;体视镜下Willis环充盈、立体。结论通过小鼠右侧颈总动脉途径灌注Microfil显示Willis环方法简单、可行,不仅能使Willis环充盈良好,还能清晰显示其立体结构,具有MRA成像效果。

Objective To evaluate the feasibility and efficacy of Microfil perfusion through right common carotid artery to visualize Willis circle in experimental mice. Methods Twenty male C57 BL/6 mice（about 14 weeks of age and 20 g of weight） were selected. The mice were heparinized and fixed after general anesthesia, then the thoracic cavity was surgically opened. The saline, 4% paraformaldehyde solution, and2% glutaraldehyde solution were perfused in turn into left ventricle from the apical part of the mice. After separating right common carotid artery, Microfil was perfused through the detaining needle in left ventricle that was inserted into the proximal end of right common carotid artery, the mice were stored at 4°C overnight,then their brains were removed and the Willis circle of each mouse was examined under stereomicroscope.Based on the stereomicroscopic findings, Willis circle could be classified into three types： totally filling,largely filling and poorly filling. Results Successful Microfil perfusion was accomplished in all 20 mice. Of the 20 mice, totally filling was observed in 11（55%）, largely filling in 4（20%）, and poorly filling in 5（25%）. Gross observation showed that Willis circle was complete, the stained blood vessel was clear; under stereomicroscope Willis circle showed well-stacked with stereoscopic effect. Conclusion For the visualization of Willis circle of experimental mice, Microfil perfusion through right common carotid artery is a simple and feasible method. This technique can not only make the Willis circle well filled, but also clearly display its stereoscopic structures, having the effect of MRA imaging.