套袋促进茌梨果实色素的合成及上调PbcrtZ基因的表达

Bagging on Pigment Synthesis and Expression of PbcrtZ in 'Chili' Fruit

通过测定花后不同时期套袋茌梨果实的外观品质和贮藏期间果实的失重率,确定茌梨最佳套袋时期为花后60 d。以花后60 d套袋的茌梨果实为试材,分析了塑膜袋和无纺布袋处理的果实果皮中叶绿素含量、类黄酮含量和类胡萝卜素含量以及色泽相关差异基因PbcrtZ基因的表达模式。套塑膜袋和无纺布袋的茌梨果实发育阶段的叶绿素含量明显低于未套袋果实,而类黄酮含量以及类胡萝卜素含量在花后180 d明显高于未套袋果实。PbcrtZ基因在套塑膜袋和无纺布袋果实中的表达明显高于未套袋果实,花后180 d分别为对照的4.36倍和3.48倍,与类胡萝卜素含量和类黄酮含量差异一致。因此,塑膜袋和无纺布套袋果实色泽呈黄绿色,未套袋果实呈现绿色,其中无纺布袋处理的果实外观品质和贮藏性明显优于塑膜袋套袋果实。

The best bagging time of Chili pear was 60 d after anthesis by determining the appearance quality and the weight loss rate during the storage period. The Chili pear fruit, which was bagged at 60 d after anthesis, was used as the material, and the contents of chlorophyll, flavonoids, carotenoids and the expression of the color-related difference gene. PbcrtZ were determined in fruit peels treated with plastic fabrics and non-woven bags. The results showed that the content of chlorophyll in non-woven fabric and plastic bagged developmental Chili fruits was significantly lower than that in non-bagged fruits, and the contents of flavonoid and carotenoid were significantly higher than those of non-bagged fruits on 180 d after anthesis. In addition, the expression of PbcrtZ in non-woven fabric and plastic bagged fruits were higher than that in non-bagged fruits, and the expression on 180 d after anthesis was 4.36 and 3.48 times higher than that of the control group, which was in according with the difference of flavonoid content and carotenoid content. Consequently, the color of the fruits treated with non-woven fabrics and plastic bags was green-yellow and the non-bagged fruit showed a green color, and the fruits treated with non-woven fabrics showed better appearance quality and storage property.