细胞活性氧荧光探针研究现状

Current development of fluorescent probes for cellular reactive oxygen species

活性氧(reactive oxygen species,ROS)在机体多种病理生理过程中发挥重要作用,根据ROS种类、含量和亚细胞定位的不同可产生不同的效应。特异性地量化和定位ROS对深入了解其在不同病理生理过程中的作用具有重要意义。目前,已研发多种荧光探针并广泛用于标定细胞内ROS。然而,由于技术手段的限制,这些探针在特异性及稳定性等方面还存在一定缺陷。在ROS相关研究中,如不能正确认识这些探针的特性、缺陷并合理选择,可能会对研究结果造成影响。该文对现有细胞ROS荧光探针的优劣进行总结,并重点分析了线粒体靶向探针的特性,为ROS相关研究的开展提供参考。

Reactive oxygen species （ROS） plays an important role in physiological and pathological processes in cells. Different effects can be produced depending on the type and subcellular localization of ROS. Therefore, various fluorescent probes have been developed to quantify and localize ROS specifically. However, due to technical limitations, these probes have defects in specificity and stability. In this paper, we review existing cellular and mitochondrial ROS probes, and summarize their characteristics.