桂枝、炙甘草剂量变化对桂枝甘草汤药效组分的影响

Influence of dose changes of Cinnamomi Ramulus and honey-fired Glycyrrhizae Radix on active components alignments in Guizhi Gancao Decoction

目的研究桂枝或炙甘草剂量变化对桂枝甘草汤药效组分的影响。方法采用HPLC-DAD多波长切换法同时测定10种成分的变化。Hypersil Gold-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈–0.1%磷酸溶液,梯度洗脱;检测波长230 nm(苯甲酸钠、甘草苷、甘草素)、254 nm(桂皮醇、甘草酸)、276 nm(香豆素、桂皮酸、桂皮醛)、370 nm(异甘草苷、异甘草素);体积流量0.8 mL/min;柱温20℃;进样量20μL。考察桂枝或炙甘草剂量变化对各药材、桂枝甘草汤药效组分的影响。结果适当增加桂枝(炙甘草)剂量可促进炙甘草(桂枝)药效组分的溶出,当桂枝(炙甘草)超过一定剂量,炙甘草(桂枝)药效组分的含量反而降低。桂枝、炙甘草剂量变化时其自身的药效组分含量变化明显。随着桂枝或炙甘草剂量的增加,桂枝(炙甘草)5种药效组分总量均呈先增后减的趋势,当配伍剂量与经方相一致时有最大值。结论桂枝(炙甘草)剂量变化对炙甘草(桂枝)药效组分、自身药效组分及药效组分总量均有影响,提示桂枝甘草汤用药剂量科学合理。

Objective To study the influence of Cinnamomi Ramulus and honey-fired Glycyrrhizae Radix dose changes on active components alignments in Guizhi Gancao Decoction. Methods Ten components were simultaneously determined by HPLC-DAD with changing ultraviolet-visible wavelength method. Hypersil Gold-C（18）（250 mm × 4.6 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution, with a flow rate of 0.8 mL/min. The column temperature was set at 20 ℃, and injection volume was 20 μL. The detection wavelength was set at 230 nm（sodium benzoata, liquiritin, and linquiritigenin）, 254 nm（cinnamic alcohol and glycyrrhizic acid）, 276 nm（coumarin, cinnamic acid, and cinnamaldehyde）, and 370 nm（isoliquiritin and isolinquiritigenin）. Dose changes of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix on active components in Chinese herbal medicine in prescription and Guizhi Gancao Decoction were investigated. Results The dosage of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix could promote the dissolution of the active components. When Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix exceeded a certain dose, the contents of active components were decreased. When dosages of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix changed, their active components had obvious changes. With the increase of the dose of Cinnamomi Ramulus and honey-fired Glycyrrhizae Radix, the total amounts of the five active components increased first and then decreased, and when the compatibility dose was the same as that of Guizhi Gancao Decoction prescription, they reached the maximum. Conclusion The dose change of Cinnamomi Ramulus or honey-fired Glycyrrhizae Radix has influence on the active components, itself active components, and total active components, and it indicates that the dosage of Guizhi Gancao Decoction is scientific and reasonable.