利用de novo测序分析蚕豆瓣酱醅微生物多样性

Analysis of Microbial Diversity in Doubanjiang-meju Using de novo Sequencing Method

【目的】分析蚕豆瓣醅微生物多样性并比较与过去采用16S、ITS等扩增子测序的差异。【方法】利用de novo测序方法研究微生物菌群多样性。【结果】嗜盐四联球菌(Tetragenococcus halophilus)是蚕豆瓣酱醅的优势微生物,相对丰度占一半以上(m OTUs方法为64.26%,Metaphlan2方法为50.80%),Metaphlan2方法发现破布子乳酸菌(Lactobacillus pobuzihii)也是优势菌株,其相对丰度为17.60%,另外,存在大量未分离到的微生物有待进一步研究。【结论】该方法能分析到蚕豆瓣醅微生物中种或菌株的水平,研究结果不仅为后续深入研究提供了技术支撑,而且对郫县豆瓣的微生物安全性评价提供了有效的方法。

[Objective]The study aimed to analyze the microbial diversity in Doubanjiang-meju using the de novo genome sequencing and compare with amplicon sequencing methods. [ Method] de novo genome sequencing. [ Result] The results showed that de novo method could analyze at species or strain level. Tetragenococcus halophilus was the predominant species（above 50 % relative abundance） and Lactobacillus pobuzihii was also predominant species （17.60 %）. [Conclusion] Previous amplicon sequencing methods, such as 16S and ITS, of which accuracy is limited, often only to genus, while de novo sequencing has the ability to compensate for these shortcomings, with being accurate to species or strain level. The results not only provide technical support for further research, but also provide an effective method for assessment of microbial safety in Doubanjiang.