摘要
Bacillus subtilis HAS had good control effects on sugarcane smut in the field. Colonization dynamics of B. subtilis HAS were studied through biotics marker and pot culture, to understand the bio-control mechanism of the strain and provide experimental basis for commercialization. The results showed that B.subtilis HAS had strong colonization ability, which could be colonized in sugarcane root, stem, leaf and rhizosphere for long time, but the colonization levels were different. The colonization density of B.sutili HAS remained 103 CFU/g soil in the rhizosphere and 102 CFU/g （fresh weight） in the root after inoculation of 60 d. The colonization in the rhizosphere decreased first, then increased and decreased again, and finally stabilized. The colonization in the root first increased then decreased. Meantime, colonization status in stems and leaves showed that the colonization density in stems and leaves was 10 CFU/g （fresh weight）, indicating that the colonization level was not high in stem and leaf.
Bacillus subtilis HAS had good control effects on sugarcane smut in the field. Colonization dynamics of B. subtilis HAS were studied through biotics marker and pot culture, to understand the bio-control mechanism of the strain and provide experimental basis for commercialization. The results showed that B.subtilis HAS had strong colonization ability, which could be colonized in sugarcane root, stem, leaf and rhizosphere for long time, but the colonization levels were different. The colonization density of B.sutili HAS remained 103 CFU/g soil in the rhizosphere and 102 CFU/g （fresh weight） in the root after inoculation of 60 d. The colonization in the rhizosphere decreased first, then increased and decreased again, and finally stabilized. The colonization in the root first increased then decreased. Meantime, colonization status in stems and leaves showed that the colonization density in stems and leaves was 10 CFU/g （fresh weight）, indicating that the colonization level was not high in stem and leaf.
基金
Supported by National Natural Science Foundation of China(31471555)
Basic Scientific Research Fund of Central Public Welfare Research Institutes(1630052016010)
China Agricultural Industry Research System(CARS-170301)
