DCLK1、Bmi-1在结直肠癌中的表达及临床意义

Expression of DCLK1 and Bmi-1 in colorectal cancer and their clinical significance

目的探讨双肾上腺素样激酶1(DCLK1)和B细胞特异性莫洛氏鼠白血病病毒插入位点-1(Bmi-1)在结直肠癌(CRC)组织中的表达及临床意义。方法采用免疫组化Max Vision法检测DCLK1和Bmi-1在74例CRC组织及对应的癌旁组织中的表达情况,分析两者表达的相关性及其与临床病理特征的关系。结果 CRC组织中DCLK1和Bmi-1的阳性表达率分别为68.9%和62.2%,高于癌旁正常组织中的27.0%和23.0%,差异均有统计学意义(P<0.05)。DCLK1表达与浸润深度、淋巴结转移和Dukes分期有关(P<0.05);Bmi-1表达与分化程度、浸润深度、淋巴结转移和Dukes分期有关(P<0.05)。DCLK1与Bmi-1表达水平呈正相关(r=0.259,P=0.026)。结论 DCLK1、Bmi-1在CRC组织中高表达,其阳性表达可能与CRC的发生、发展密切相关,两者联合检测可能为CRC的临床诊治及预后评估提供新途径。

Objective To investigate the expression of doublecortin-like kinase 1（DCLK1） and B-cell specific moloney leukemia virus insertion site 1（ Bmi-1） in colorectal cancer and their clinical significance. Methods The expression of DCLK1 and Bmi-1 were detected in the 74 cases of colorectal cancer and the corresponding adjacent normal tissues by Max Vision method. The correlation between the expression of two protein and their relationship with clinical pathological parameters of colorectal cancer were analyzed. Results The positive expression rates of DCLK1 and Bmi-1 in colorectal cancer were 68. 9% and 62. 2%,higher than 27. 0%and 23. 0% in their adjacent normal tissues respectively,and the differences were significant（ P〈0. 05）. DCLK1 was significantly correlated with infiltration＇s depth,lymphatic metastasis and Dukes＇ stage in colorectal cancer（ P〈0. 05）. Bmi-1 was signifcantly correlated with tumor classification,infiltration＇s depth,lymphatic metastasis and Dukes＇ stage（ P〈0. 05）. The expression of DCLK1 was positively correlated with that of Bmi-1（ r = 0. 259,P = 0. 026）. Conclusion DCLK1 and Bmi-1 were highly expressed in colorectal cancer tissues and associated with the development and progression of colorectal cancer. The joint detection of them may provide a new approach for the clinical diagnosis,treatment and prognostic evaluation of colorectal cancer.