摘要
目的:探讨IGF-1基因修饰的肌源性干细胞(MDSCs)移植治疗雌性压力性尿失禁大鼠的作用及可能机制。方法:尿失禁大鼠随机分对照组即MDSCs移植组(n=15)和治疗组即IGF-1基因修饰的MDSCs移植组(n=15),以1×10~6个MDSCs/只注入尿道距膀胱约0.5 cm处,评价注射后尿道闭合功能情况、VEGF的表达情况及移植细胞存活情况。结果:与对照组比较,治疗组膀胱最大容量及腹压漏尿点压(ALPP)均升高,但差异无统计学意义(P>0.05);细胞存活数、VEGF蛋白表达量显著升高,差异有统计学意义(P<0.05)。结论:IGF-1基因修饰的MDSCs增强了其在移植环境中的生存能力,并通过其旁分泌功能,可能为治疗压力性尿失禁提供了一种新的方法。
Objective：To explore that whether muscle-derived stem cells（MDSCs） pretreated with IGF-1 could promote urethral sphincter restoration in pudendal nerve-transected rat model.Method：Rats with Female Stress Urinary Incontinence were randomly divided into IGF-1/MDSCs injection group（the experimental group,n=15） and MDSCs injection group（the control group,n=15）.MDSCs survival,urethral resistance function,western blot were carried out.Result：Compared with the control group,the maximum capacity of the bladder and abdominal leak point pressure（ALPP） were all increased（P〉0.05）,and the number of cells survival and expression of VEGF protein were increased significantly in the experimental group（P〈0.05）.Conclusion：MDSCs pretreated with IGF-1 that enhance their viability in the transplant environment and through their paracrine function may provide a new way to treat stress urinary incontinence.
作者
陈春经
CHEN Chunjing(Sanming First Hospital Affiliated to Fujian Medical University,Sanming 365000,Chin)
出处
《中外医学研究》
2018年第14期170-172,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
基金
福建省自然基金项目(项目编号:2014J05100)
三明市科技资助项目(项目编号:2013-S-3)
