普通油茶花发育过程数字表达谱测序及分析

Sequencing and Analysis of Digital Expression Profiles of Camellia oleifera Flower Bud Differentiation

为了解普通油茶花发育过程的分子机制及成花关键基因,选择花芽分化前、萼片形成期、花瓣形成期、雌雄蕊形成期、子房及花药形成期和雌雄蕊成熟期的花芽作为试验材料,对此6个时期样品进行表达谱测序并进行分析。结果表明,测序共获得原始reads数为103 249 386条,平均Cycle Q20均为100%;各样品测序reads与转录组部分测序构建的Unigene库的序列对比,对比效率超过60%,共获得差异表达基因26 861个,7月3日与6月15日差异基因达到19 596个,上调基因数19 408个;对差异基因进行生物功能分析、翻译,核糖体结构和生物合成为245条,转录因子为372条,复制、重组和修复为320条,细胞信号转导机制为285条,翻译后修饰、蛋白质周转和伴侣蛋白为283条,能源生产和转换为114条,碳水化合物运输和代谢为136条,氨基酸的转运和代谢为122条;与花器官形成有关A类基因Unigene数量最多,其中Unigene67783在不同的发育阶段差异较明显,表达水平也较高;B类基因有9条Unigene,其中Unigene56059表达水平较高,各发育阶段差异也较明显;属于C类基因的Unigene数量有9条,总体表达水平较低且差异不明显。

In order to understand the molecular mechanism of the development of Camellia oleifera and the key genes of flowering,the flower buds were selected at six different stages（ before flower bud differentiation,sepal formation,petal formation,male and female stoma formation,ovary and anther formation,and male and female maturation）,the samples of six stages were sequenced and analyzed. The results showed that： sample sequencing from six stages received a total of 103 249 386 raw reads which the average Cycle Q20 was 100%. The samples sequenced reads were used to compare with transcriptome sequences and Unigene library,and the comparative efficiency was more than 60%; By analyzing the differences genes between all samples,the differentially expressed genes were 26 861 in total,more differentially expressed genes（ 19 596 genes） were found in the samples from July 3 rdand June 15 th,the number of up-regulated genes were 19 408. All differentially expressed genes were analyzed on biological function,and translated,it was found that the number of ribosome structure and biosynthesis was 245,the transcription factor was 372,the replication,recombination and repair was 320,the cell signal transduction mechanism was 285,the post-translational modification,the protein turnover and the companion protein was 283,the energy produced and converted was 114,the carbohydrate transported and metabolized was 136,the amino acid transported and metabolized was 122. The number of A-type gene Unigene,which is related to the formation of floral organ presented the most,among which,Unigene 67783 varied greatly in all developmental stages,and the expression level of Unigene 67783 was higher than that of others. The expression level of Unigene56059 was higher in the B-type gene,and the difference was also higher obviously. The number of C-type gene Unigene was 9,the overall expression level was low and the difference was not obvious.