银屑病患者辅助性T细胞17和调节性T细胞及相关细胞因子检测的临床意义

Clinical significances of helper T cell 17,regulatory T cell and the expression of relative cytokines in psoriasis patients

目的探讨银屑病患者外周血辅助性T细胞17(Th17)、调节性T细胞(Treg)及相关细胞因子检测的临床意义。方法选取2016年1-12月解放军第四○四医院皮肤科收治的银屑病患者60例(银屑病组)及健康体检者30例(对照组),应用流式细胞仪检测外周血中Th17和Treg比例,应用酶联免疫吸附试验方法测定Th17、Treg相关细胞因子白细胞介素(IL)17、IL-10水平。结果银屑病组Th17比例高于对照组,Treg比例低于对照组,Th17/Treg比值明显高于对照组[(2.03±0.27)%比(0.80±0.32)%、(1.10±0.21)%比(2.80±0.43)%、(1.91±0.46)比(0.30±0.14)],差异均有统计学意义(均P<0.05)。银屑病组血清IL-17水平明显高于对照组,IL-10水平明显低于对照组[(101±11)ng/L比(56±8)ng/L、(16±3)ng/L比(30±6)ng/L],差异均有统计学意义(均P<0.05)。结论银屑病患者中存在Th17及Treg免疫平衡失调。

Objective To investigate the clinical significances of helper T cell 17 （Th17）, regulatory T cell （Treg） and the expression of relative cytokines in psoriasis patients. Methods From January to December in 2016, 60 psoriasis patients（psoriasis group） and 30 healthy people（control group） were enrolled in 404 Hospital of the Chinese People＇s Liberation Army. Levels of Th17 and Treg were detected by flow cytometry; serum concen- trations of interleukin（IL）-17 and IL-10 were detected by enzyme-linked immunosorbent assay. Results Th17percentage in psoriasis group was higher than that in control group; Treg percentage in psoriasis group was lower than that in control group; Th17/Treg ratio in psoriasis group was higher than that in control group [ （2. 03 ±0.27）% vs （0.80±0.32）%, （1.10±0.21）% vs （2.80±0.43）%, （1.91 ±0.46） vs （0.30±0.14）1（P〈 0. 05）. Serum IL-17 level in psoriasis group was higher than that in control group and serum IL-10 level in psoriasis group was lower than that in control group[ （101 ± 11）ng/L vs （56±8）ng/L, （16 ±3） ng/L vs （30±6） ng/L] （P 〈 0. 05 ）. Conclusion Psoriasis patients may have Th17 and Treg immune balance disorder.