新疆青杞的化学成分分离鉴定

Chemical Constituents Identification from Solanum septemlobum

目的：研究新疆青杞全草的化学成分。方法：利用硅胶柱色谱,LH-20羟丙基葡聚糖凝胶（Sephadex LH-20）柱色谱及高效制备薄层色谱等方法对新疆青杞95%乙醇提取物的乙酸乙酯部位进行分离纯化,依据理化性质和波谱学数据对其化学结构进行阐明。结果：从乙酸乙酯部位发现21个化合物,包括3个酰胺,7个酚酸,1个香豆素及10个黄酮类化合物,分别为N-反式香豆酰基酪胺（1）,N-3-羟基-4-甲氧基苯乙基反式阿魏酸酰胺（2）,对羟基苯甲酸（3）,藜芦酸（4）,原儿茶酸（5）,3,4-二羟基苯甲酸甲酯（6）,邻羟基苯甲酸（7）,对羟基苯甲酸乙酯（8）,3,4-二羟基苯甲酸乙酯（9）,七叶内酯（10）,甘草素（11）,橙皮素（12）,柚皮素（13）,木犀草素（14）,芹菜素（15）,柯伊利素（16）,5,7-二羟基-3＇,4＇-二甲氧基黄酮（17）,香叶木素（18）,大豆苷元（19）,染料木素（20）,N-反式阿魏酰基酪胺（21）。结论：化合物1-20为首次从青杞中发现,化合物3-12,14-17为首次从茄属植物中发现。经文献调研发现,分离得到的酚酸及黄酮类化合物具良好抗炎、抗氧化活性,为进一步开展新疆青杞其药效物质基础研究及其开发利用提供参考依据。

Objective： To investigate the chemical constituents of Solanum septemlobum. Method： The ethyl acetate fraction of 95% ethanol extract of S. septemlobum was isolated and purified by silica gel column chromatography,Sephadex LH-20 and high performance thin layer chromatography(HPTLC）. The structures were identified by physico-chemical properties and spectral data. Result： Twenty-one compounds were isolated and purified as： N-trans-coumaroyltyramine(1）,N-trans-feruloyl-4＇-O-methyldopamine(2）,p-hydroxybenzoic acid(3）,3,4-dimethoxy benzoic acid(4）, protocatechuate(5）, methyl 3,4-dihydroxybenzoate(6）,2-hydroxybenzoic acid(7）,ethyl 4-hydroxybenzoate(8）,ethyl 3,4-dihydroxybenzoate(9）,esculetin(10）,liquiritigenin(11）,hesperetin(12）,naringenin(13）,luteolin(14）,apigenin(15）,hrysoeriol(16）,diosmetin(17）,5,7-dihydroxy-3＇,4＇-dimethoxyflavone(18）, isoliavoues(19）,genistein(20）, and Ntrans-feruloyltyramine(21）. Conclusion： Compounds 1-20 were isolated from S. septemlobum and compounds 3-12 and 14-17 were obtained from Solanum for the first time. The literature review showed that amides and flavonoids had anti-inflammatory and antioxidant activities, providing a reference for further studies on pharmacodynamic material basis and the development and utilization of S. septemlobum from Xinjiang.