基于网络药理学的茵陈五苓散作用机制分析

Analysis on Mechanism of Yinchen Wulingsan Based on Network Pharmacology

目的：通过网络药理学方法初步探讨茵陈五苓散的作用机制。方法：本研究基于中药系统药理学技术平台、蛋白数据库和String数据库,分别获取茵陈五苓散中化学成分,及其对应靶点、疾病并构建化合物-靶点,靶点-疾病网络,蛋白相互作用网络,通过生物学信息注释数据库（DAVID）注释数据库对靶点进行基因本体生物学过程（gene ontology biology process,GO-BP）富集分析及基因组百科全书（Kyoto Encyclopedia of Genes and Genomes,KEGG）通路分析。结果：共获得58个候选化合物,103个靶点,11个中枢（hub）靶点及262种疾病,关键靶点涉及肿瘤坏死因子（TNF）,过氧化物合成酶2（PTGS2）,ESR1等,疾病以肿瘤、免疫和内分泌为主。GO-BP条目146条,参与信号转导、转录翻译、促增殖抑凋亡、细胞组成和氧化应激条目等5种生物过程。KEGG信号通路108条,含靶点数目较多的信号通路主要涉及肿瘤、病毒、寄生虫等。结论：本研究系统地探讨了茵陈五苓散的候选成分所对应的靶点及作用机制,为今后的临床研究及进一步的开发为新药提供了思路。

Objective： To investigate the therapeutic mechanism of Yinchen Wulingsan based on network pharmacology. Method： Based on the Traditional Chinese Medicine Systems Pharmacology Database,the Protein Databank and the String database,the chemical compositions of Yinchen Wulingsan,corresponding targets and diseases were obtained respectively. Meanwhile,compound-target network,as well as target-disease network and protein-protein interaction network,were constructed. According to biological information annotation dotabase(DAVID） annotation databases, gene ontology biology process(GO-BP） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG） pathway analysis of the targets were performed. Result： Totally 58 components,103 targets,11 hub targets and 262 diseases were obtained. The key targets involved tumor necrosis factor(TNF）,peroxidase synthetase 2(PTGS2）,and estrogen receptor(ESR1）,etc. The diseases mainly involved tumor,immune and endocrine. There were 146 GO-BP entries,including signal transduction entries,transcription and translation entries,proliferation promotion and apoptosis inhibition entries,cellular component entries,and oxidative stress entries. Besides,there were 108 KEGG pathways,involving tumor,virus,parasite and so on. Conclusion： The targets and mechanism corresponding to compounds of Yinchen Wulingsan were studied systematically in this study,providing ideas for further clinical researches and new drug development.