摘要
The customized implants which are composed of polycaprolactone( PCL) and strontium substituted nanohydroxyapatite( SrHA) were fabricated successfully by using fused deposition modeling( FDM),which is a simple 3 D printing technology for fabricating personalized products. The physical and chemical properties of composite scaffolds were characterized by transmission electron microscopy( TEM), Fourier transform infrared spectroscopy( FTIR), X-Ray diffraction( XRD) and inductively coupled plasma-atomic emission spectroscopy( ICPAES). The results suggested that strontium element was successfully doped into nanohydroxyapatite and all scaffolds showed the homogeneous network structure. Furthermore, the in vitro biocompatibility of the scaffolds was evaluated by cell counting kit-8( CCK-8) assay. The data indicated that the prepared scaffolds exhibited excellent biocompatibility to bone marrow mesenchymal stem cells( BMSCs). Besides,strontium element can be released from PCL-SrHA scaffolds in a sustained manner. Therefore,the 3 D printing PCL-SrHA scaffolds hold great potential for bone tissue engineering.
The customized implants which are composed of polycaprolactone（ PCL） and strontium substituted nanohydroxyapatite（ SrHA） were fabricated successfully by using fused deposition modeling（ FDM）,which is a simple 3 D printing technology for fabricating personalized products. The physical and chemical properties of composite scaffolds were characterized by transmission electron microscopy（ TEM）, Fourier transform infrared spectroscopy（ FTIR）, X-Ray diffraction（ XRD） and inductively coupled plasma-atomic emission spectroscopy（ ICPAES）. The results suggested that strontium element was successfully doped into nanohydroxyapatite and all scaffolds showed the homogeneous network structure. Furthermore, the in vitro biocompatibility of the scaffolds was evaluated by cell counting kit-8（ CCK-8） assay. The data indicated that the prepared scaffolds exhibited excellent biocompatibility to bone marrow mesenchymal stem cells（ BMSCs）. Besides,strontium element can be released from PCL-SrHA scaffolds in a sustained manner. Therefore,the 3 D printing PCL-SrHA scaffolds hold great potential for bone tissue engineering.
作者
刘顶华
聂伟
陈良
王伟忠
陶玲
杜海波
何创龙
LIU Dinghua, NIL Wei, CHEN Liang, WANG Weizhong, TAO Ling, DU Haibo, HE Chuanglong(College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, Chin)
基金
National Natural Science Foundations of China(Nos.31771048,31570984,31271028)
International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality,China(No.15540723400)
Innovation Foundation of Donghua University,China(No.EG2017012)
Biomedical Textile Materials Science and Technology(111 Project),China(No.B07024)
