摘要
目的 分析2016年云南省德宏傣族景颇族自治州(德宏州)新报告HIV感染者HIV基因亚型,了解中国籍和缅甸籍HIV感染者基因亚型分布特征。方法 对2016年德宏州新报告的HIV感染者提取核酸,使用RT-PCR法对HIV基因gag、env和pol进行反转录扩增,直接测序扩增产物,综合分析3个基因的测序结果,确定最终基因亚型。结果 2016年德宏州新报告HIV感染者1 112例,成功测定基因亚型的感染者860例。C亚型所占比例最大(占33.6%,289/860);其后为独特重组(unique recombinant forms,URFs)(占28.4%,244/860)、CRF01_AE(占18.6%,160/860)等亚型;URFs中主要包括4种形式的重组,其中以CRF01_AE和C亚型重组为主。缅甸籍感染者的亚型分布与民族和传播方式相关。结论 德宏州2016年新报告HIV感染者基因亚型主要集中在C、URFs和CRF01_AE亚型,URFs分布依然复杂多样。
Objective To explore distribution of HIV gene subtypes among newly reported HIV/AIDS cases from China and Myanmar in Dehong Dai and Jingpo prefecture of Yunnan province in 2016. Methods We conducted DNA extractions from newly reported HIV/AIDS cases in 2016. The gag, env and pol genes were amplified by using reverse transcription-PCR (RT-PCR) and sequenced to identify HIV subtypes. Results A total of 1 112 newly diagnosed HIV cases were reported in Dehong in 2016, and the HIV subtypes were identified for 860 cases. Subtype C was predominant (33.6%), followed by unique recombinant forms (URFs) (28.4%), CRF01_AE (18.6%) and so on. URFs include four recombination, among which the recombination of CRF01_AE and C subtype were predominant. The HIV subtype distribution was associated with nationality and transmission route in HIV/AIDS cases from Myanmar. Conclusions The gene subtypes of C, URFs and CRF01_AE were mainly distributed; distribution of URFs remained complex and diverse among newly reported HIV/AIDS cases in Dehong in 2016.
作者
段星
王可然
王继宝
叶润华
王译葵
杨锦
杨涛
周素娟
杨跃诚
姚仕堂
段松
何纳
Duan Xing, Wang Keran, Wang Jibao, Ye Runhua. Wang Yikui, Yang Jin, Yang Tao, Zhou Sujuan, Yang Yuecheng, Yao Shitang, Duan Song, He Na(Dehong Dai and Jingpo Autonomous Prefectural Center for Disease Control and Prevention, Mangshi 678400, China; Department of Epidemiology, School of Public Health, Key Laboratory for Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, Chin)
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2018年第5期678-681,共4页
Chinese Journal of Epidemiology
基金
国家重点地区艾滋病防治项目;国家科技重大专项(2013ZX10004-906);国家自然科学基金(81373062)