两种食物胆固醇检验方法对居民膳食胆固醇摄入量评估的影响

Influence of the two dietary cholesterol testing methods on the dietary cholesterol intake assessments

目的研究食物胆固醇检测技术对居民膳食胆固醇摄入量评估结果的影响,为科学评估胆固醇的膳食摄入量提供依据。方法采用超高效液相色谱法(UPLC)和分光光度法对肉类、奶类、蛋类食物分别进行检测,以2010-2012年中国居民营养与健康状况监测(CNHNS)北京市的膳食调查数据为基础,用两种方法检测得到的食物胆固醇含量数据计算人群膳食胆固醇摄入量,评估检测方法的改变对居民膳食胆固醇摄入量的影响。结果两种食物胆固醇检验方法在肉类和奶类中胆固醇检测数据差异无统计学意义(P>0.05),在蛋类中胆固醇检测数据差异有统计学意义(P<0.05)。采用超高效液相色谱法(UPLC)检测得到的食物胆固醇含量数据计算人群膳食胆固醇摄入量中位数为239 mg/d,采用分光光度法检测得到的食物胆固醇含量数据计算人群膳食胆固醇摄入量中位数为205 mg/d,两者差异有统计学意义(P<0.05)。结论关注检验方法的变化对居民膳食胆固醇摄入量的影响是十分必要的。

Objective To analyze the effects of the dietary cholesterol testing technology on the assessment of dietary cholesterol intake,so as to provide the basis for scientific evaluation of cholesterol dietary intake. Methods Both ultra high performance liquid chromatography（ UPLC） and spectrophotometry were used to test meat,milk and eggs,respectively. Results of cholesterol contents obtained by both testing method in Beijing dietary survey of the 2010 to 2012 Chinese residents of nutrition and health status monitoring（ CNHNS） were applied to calculate population dietary cholesterol intake,and to evaluate the influence by both method on dietary cholesterol intake among residents. Results The result of both cholesterol testing method in meat and dairy food showed no statistically significant difference（ P〉0. 05）,however the difference of cholesterol in eggs by both method was statistically significant（ P〈0. 05）. The median value of dietary cholesterol intake by UPLC was 239 mg/d while the value obtained by. spectrophotometry was 205 mg/d. The difference was statistically significant（ P〈0. 05）.Conclusion Bias on dietary cholesterol intake caused by applying different dietary cholesterol testing should be concerned.