广西普通野生稻群体结构解析与核心种质构建

Genetic Structure and Core Collection of Common Wild Rice(Oryza rufipogon Griff.) in Guangxi

利用覆盖水稻12条染色体的64个分子标记,对广西境内已发现的283个野生稻自然居群按居群取样原则采集4173份代表性样本进行遗传结构分析并构建核心种质。结果显示,64个标记位点共检测出1180个等位变异,平均等位变异数为18.4375,Shannon指数为1.7367,Nei's多样性指数0.7182,表明广西普通野生稻资源遗传多样性十分丰富。同时,基于广西普通野生稻群体结构,构建了包含351份种质的广西普通野生稻核心种质,占原样本数的8.41%。广西普通野生稻核心种质,代表广西普通野生稻的多样性和特异性,为野生稻遗传资源的深入研究提供基础,从而为水稻育种提供应用信息。

To explore genetic diversity and population gion in China,we investigatedthe genetic diversity of 4173 structure of common wild rice in Guangxi Autonomous Re- common wild rice germplasms that were collected from 14 cities in Guangxi, by using 64 molecular markers on 12 chromosomes. A total of 1180 alleles was detected （ mean of Al- leles Per Locus of 18. 4375 ,mean of Shannon index of 1.7367,and mean of Nei＇s diversity index of 0. 7182）. With that, the result indicated the higher genetic diversity of common wild rice in Guangxi. We suggested a core collection of 351 germplasms （8. 41% in total collection）using priority preservation-stepwise cluster method. This core collection of com- mon wild rice could represent the diversity and specificity of common wild rice in Guangxi, and thus this resource be- comes valuable in in-depth study in the wild rice genetic resources and hence utilization of variation in rice breeding.